規(guī)格：96t/48t

大鼠熱休克蛋白糖蛋白96試劑盒

原理：ELISA是以免疫學(xué)反應(yīng)為基礎(chǔ)，將抗原、抗體的特異性反應(yīng)與酶對底物的高效催化作用相結(jié)合起來的一種敏感性很高的試驗(yàn)技術(shù)。由于抗原、抗體的反應(yīng)在一種固相載體──聚苯乙烯微量滴定板的孔中進(jìn)行，每加入一種試劑孵育后，可通過洗滌除去多余的游離反應(yīng)物，從而保證試驗(yàn)結(jié)果的特異性與穩(wěn)定性。在實(shí)際應(yīng)用中，通過不同的設(shè)計(jì)，具體的方法步驟可有多種。即:用于檢測抗體的間接法(圖a)、用于檢測抗原的雙抗體夾心法(圖b)以及用于檢測小分子抗原或半抗原的抗原競爭法等等。比較常用的是ELISA雙抗體夾心法及ELISA間接法。

大鼠熱休克蛋白糖蛋白96試劑盒

B403 蘋果酸酶（ME）試劑盒 紫外分光光度法 50管/48樣

大鼠熱休克蛋白糖蛋白96試劑盒

Principle: ELISA is a highly sensitive immunological reaction test based technique, the antigen, the antibody specifically reacts with the enzyme substrate for efficient catalysis combined together. Since the antigen antibody reaction in a solid phase carrier ─ ─ polystyrene microtiter plate wells conducted after each addition of a reagent incubation, the excess can be removed by washing the free reactants to ensure the specificity of the test results and stability. In practice, the different design of specific process steps can have many. Namely: the indirect method for the detection of antibodies (Figure a), for the detection of antigen double antibody sandwich method (Figure b) and small molecules for the detection of antigen or hapten antigen competition law, and so on. More common is the double antibody sandwich ELISA and indirect ELISA.