bio-rad伯樂(lè)/bio-rad轉(zhuǎn)基因系統(tǒng)
產(chǎn)品名稱:Helios® 基因槍系統(tǒng) bio-rad伯樂(lè)/bio-rad轉(zhuǎn)基因系統(tǒng)
Helios 基因槍是一種方便的手提式設(shè)備,可對(duì)細(xì)胞進(jìn)行快速直接的原位基因轉(zhuǎn)移。該系統(tǒng)通過(guò)可調(diào)節(jié)的氦氣脈沖,來(lái)帶動(dòng)位于小塑料管內(nèi)壁,包被有 DNA、RNA 或其它生物材料的金粉顆粒,將其直接打入細(xì)胞內(nèi)部。使用樣品管制備站可很容易地制備管內(nèi)子彈。
主要功能
簡(jiǎn)單、快速、功能靈活的基因傳送,各種細(xì)胞通用
適用于瞬時(shí)表達(dá)和穩(wěn)定表達(dá)
只需少量的 DNA 和細(xì)胞,無(wú)需載體 DNA
能進(jìn)行多質(zhì)粒共同導(dǎo)入
可輸送大片段 DNA
胞內(nèi)目標(biāo)基因可導(dǎo)入多個(gè)細(xì)胞
用于體內(nèi)和體外轉(zhuǎn)化
沒(méi)有無(wú)關(guān)的基因或蛋白被轉(zhuǎn)導(dǎo)
訂貨信息:
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貨號(hào)
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描述
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165-2411
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Helios Gene Gun Kit,100/120 V, handheld biolistic system, includes gene gun, 5 O-rings, 5 barrel liners, 5 white cartridge holders, cartridge extractor tool, 9 V battery
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165-2431
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Helios Gene Gun System,100/120 V, handheld biolistic system, includes helium hose assembly with regulator, tubing prep station (#165-2418), syringe kit, Tefzel tubing, tubing cutter, optimization kit (#165-2424)
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165-2451
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Helios Gene Gun Low-Pressure System,100/120 V, handheld biolistic system, includes helium hose assembly with low-pressure regulator, tubing prep station (#165-2418), syringe kit, tubing cutter, optimization kit (#165-2424)
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配件及耗材
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165-2264
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1.6 μm Gold Microcarriers,0.25 g, 1.6 μm diameter, gold microcarriers for use with the PDS-1000/He and Helios gene gun systems
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165-2263
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1.0 μm Gold Microcarriers,0.25 g, 1.0 μm diameter, gold microcarriers for use with the PDS-1000/He and Helios gene gun systems
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165-2262
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0.6 μm Gold Microcarriers,0.25 g, 0.6 μm diameter, gold microcarriers for use with the PDS-1000/He and Helios gene gun systems
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165-2244
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GeneShot Control Cartridges,Pkg of 12, positive control bullets, 1.6 μm gold particles coated with LacZ and luc; for use with the Helios gene gun system (#165-2431, #165-2432)
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165-2418
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Tubing Prep Station,100/120 V, Helios gene gun sample preparation station, for the preparation of microparticle cartridges
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165-2420
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Tubing Prep Station,220/240 V, Helios gene gun sample preparation station, for the preparation of microparticle cartridges
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165-2421
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Syringe Kit,Kit for loading gold suspension into Tefzel tubing, includes syringe adaptor tubing, 5 x 10 ml syringes, syringe adaptor fitting, 5 Luer-Lok fittings
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165-2422
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Tubing Cutter,Pkg of 1, instrument for cutting Tefzel tubing, for use with the Helios gene gun, includes 10 razor blades
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165-2424
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Helios Gene Gun Optimization Kit,Optimization kit for the Helios gene gun, includes 0.6 μm, 1.0 μm and 1.6 μm gold microcarriers, PVP, desiccant, 5 cartridge storage vials, 50 feet of Tefzel tubing, for 1,000 samples
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165-2440
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Cartridge Kit,Kit for preparing Helios gene gun cartridges, includes 0.5 g, PVP, 5 desiccant pellets, 5 cartridge collection/storage vials, 15 m of Tefzel tubing
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165-2416
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O-Rings,Pkg of 5, O-rings for use with the Helios gene gun system (#165-2431, #165-2432
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165-2417
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Barrel Liner,Pkg of 5, sample preparation accessory for Helios gene gun
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165-2426
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Cartridge Holder,Pkg of 5, accessory for loading microparticle-coated cartridges into the Helios gene gun, white
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165-2435
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Cartridge Extractor Tool,Pkg of 1, tool for removing discharged cartridges, for use with the Helios gene gun (#165-2431, #165-2432)
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165-2436
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Battery,Pkg of 1, 9 V alkaline battery, for use with the Helios gene gun, provides 1,000 discharges in continuous use
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165-2441
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Tefzel Tubing,15 m, tubing for coating with DNA- or RNA-gold microcarrier complexes, used with the Helios® gene gun, .125" (3.175 mm) OD, .093" (2.36 mm) ID
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165-2475
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Helios Diffusion Screens,Pkg of 5, screens for use with the Helios gene gun (#165-2431, #165-2432)
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產(chǎn)品名稱:PDS-1000 / He? 和 Hepta? 基因槍系統(tǒng) bio-rad伯樂(lè)/bio-rad轉(zhuǎn)基因系統(tǒng)
PDS-1000/He? 系統(tǒng)
PDS-1000/He 基因槍系統(tǒng)將微米大小的、包被有核酸的金或鎢微粒( 0.6–1.6 μm )加速到所需要的速度,從而將基因?qū)爰?xì)胞、組織或器官。系統(tǒng)利用高壓氦氣的轟擊,來(lái)推動(dòng)攜帶有微粒的塑料載體盤朝目標(biāo)細(xì)胞方向加速。推進(jìn)載體的氦氣壓力由所使用的不同可裂膜決定??闪涯な且环N可在特定壓力下破裂的塑料密封體。阻擋網(wǎng)可阻止載體盤,使微??梢源┻^(guò)并轉(zhuǎn)染靶細(xì)胞。
PDS-1000/He 系統(tǒng)提供:
一種可重復(fù)的、轉(zhuǎn)化完整培養(yǎng)細(xì)胞的方法,幾乎無(wú)需對(duì)細(xì)胞進(jìn)行處理
用其它方法不能完成的,有特殊生長(zhǎng)需要的細(xì)胞轉(zhuǎn)化
交互式光盤涵蓋了基因槍顆粒傳送技術(shù)進(jìn)行基因轉(zhuǎn)移的所有方面
帶有 Hepta? 適配器的 PDS-1000/He 系統(tǒng)
PDS-1000/He 基因槍系統(tǒng)加上 Hepta 適配器后,可以比標(biāo)準(zhǔn)系統(tǒng)多轉(zhuǎn)化 7-10 倍的細(xì)胞。它配接 PDS-1000/He系統(tǒng)的沖擊室,將氦氣沖擊波分導(dǎo)至 7 個(gè)載體盤處。這樣,通過(guò)在更大面積上均一地分散 DNA 包被的金粉或鎢粉,增加了這個(gè)系統(tǒng)一次轟擊的細(xì)胞數(shù)。當(dāng)氦氣被分成 7 道時(shí),降低了壓力和顆粒速度,該系統(tǒng)特別適合不太需要強(qiáng)大穿透力的植物和培養(yǎng)細(xì)胞。
技術(shù)參數(shù)
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尺寸(寬 x 深 x 高)
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29 x 25.5 x 47.5 cm
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構(gòu)造
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鋁、ABS 塑料和丙烯酸機(jī)架
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重量
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15 公斤
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輸入電壓
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100–120 VAC,50–60 Hz
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*大電流
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<5 A
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機(jī)械
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保險(xiǎn)絲
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6.3 A,250 V,5 x 20 mm
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真空
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<0.4 英寸汞/分鐘泄漏
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過(guò)壓
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0.5 psi **閥,自動(dòng)復(fù)位
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環(huán)境
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操作
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溫度: 0–35°C (32–95°F)
濕度: 0–95% 無(wú)冷凝
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儲(chǔ)存
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溫度: 0–70°C (32–158°F)
濕度: 0–95% 無(wú)冷凝
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訂貨信息:
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貨號(hào)
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描述
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165-2257
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基因槍PDS-1000/He System,100/120 V, biolistic transformation system, includes helium pressure regulator, solenoid, spacer rods, microcarrier launch assembly, target shelf, 5 macrocarrier holders, tubin
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165-2258
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基因槍PDS-1000/He Hepta System,100/120 V, biolistic transformation system, includes Hepta adaptor, helium pressure regulator, solenoid, spacer rods, microcarrier launch assembly, target shelf, 5 macrocarrier holders, tubing
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165-2225
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Hepta Adaptor for PDS-1000/He System,Pkg of 1, biolistic shock wave splitter for use with the PDS-1000/He system (#165-2257), includes 5 stopping screens
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165-2259
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變壓器Voltage Converter,Pkg of 1, voltage converter, for use with PDS-1000/He system on 220–240 V lines
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耗材
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165-2278
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500 Optimization Kit,Pkg of 1, optimization kit for the PDS-1000/He system, includes 0.25 g each of 0.6 μm, 1.0 μm, and 1.6 μm gold microcarriers, 100 each of 9 rupture disks, for 500 bombardments
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165-2335
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載體膜Macrocarriers,Pkg of 500, microparticles carrier disk, for use with PDS-1000/He and Hepta systems (#165-2257, #165-2258)
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165-2322
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載體膜支撐墊Macrocarrier Holders,Pkg of 5, reusable part, for use with the PDS-1000/He biolistic systems (#165-2257, #165-2258)
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165-2326
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可裂膜450 psi Rupture Disks,Pkg of 100, bursts at 450 psi in a partial vacuum, for use with the PDS-1000/He biolistic system (#165-2257, #165-2258)
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165-2327
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可裂膜650 psi Rupture Disks,Pkg of 100, bursts at 650 psi in a partial vacuum, for use with the PDS-1000/He biolistic system (#165-2257, #165-2258)
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165-2328
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可裂膜900 psi Rupture Disks,Pkg of 100, bursts at 900 psi in a partial vacuum, for use with the PDS-1000/He biolistic system (#165-2257, #165-2258)
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165-2329
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可裂膜1,100 psi Rupture Disks,Pkg of 100, bursts at 1,100 psi in a partial vacuum, for use with the PDS-1000/He biolistic system (#165-2257, #165-2258)
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165-2330
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可裂膜1,350 psi Rupture Disks,Pkg of 100, bursts at 1,350 psi in a partial vacuum, for use with the PDS-1000/He biolistic system (#165-2257, #165-2258)
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165-2331
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可裂膜1,550 psi Rupture Disks,Pkg of 100, bursts at 1,550 psi in a partial vacuum, for use with the PDS-1000/He biolistic system (#165-2257, #165-2258)
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165-2332
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可裂膜1,800 psi Rupture Disks,Pkg of 100, bursts at 1,800 psi in a partial vacuum, for use with the PDS-1000/He biolistic system (#165-2257, #165-2258)
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165-2333
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可裂膜2,000 psi Rupture Disks,Pkg of 100, bursts at 2,000 psi in a partial vacuum, for use with the PDS-1000/He biolistic system (#165-2257, #165-2258)
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165-2334
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可裂膜2,200 psi Rupture Disks, Pkg of 100, bursts at 2,200 psi in a partial vacuum, for use with the PDS-1000/He biolistic system (#165-2257, #165-2258)
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165-2336
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終止屏(阻擋網(wǎng))Stopping Screens, Pkg of 500, for use with the PDS-1000/He system (#165-2257)
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165-2226
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終止屏(阻擋網(wǎng))Hepta Stopping Screens, Pkg of 50, stopping screens for use with the Hepta adaptor of the PDS-1000/He systems (#165-2257, #165-2258)
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165-2262
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金粉0.6 μm Gold Microcarriers,0.25 g, 0.6 μm diameter, gold microcarriers for use with the PDS-1000/He and Helios gene gun systems
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165-2263
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金粉1.0 μm Gold Microcarriers, 0.25 g, 1.0 μm diameter, gold microcarriers for use with the PDS-1000/He and Helios gene gun systems
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165-2264
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金粉1.6 μm Gold Microcarriers, 0.25 g, 1.6 μm diameter, gold microcarriers for use with the PDS-1000/He and Helios gene gun systems
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165-2266
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鎢粉Tungsten M-10 Microcarriers, 6 g, ~0.7 μm diameter, tungsten microcarriers for use with the PDS-1000/He system
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165-2267
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鎢粉Tungsten M-17 Microcarriers
6 g, ~1.1 μm diameter, tungsten microcarriers for use with the PDS-1000/He system
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165-2268
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鎢粉Tungsten M-20 Microcarriers, 6 g, ~1.3 μm diameter, tungsten microcarriers for use with the PDS-1000/He system
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165-2269
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鎢粉Tungsten M-25 Microcarriers, 6 g, ~1.7 μm diameter, tungsten microcarriers for use with the PDS-1000/He system
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產(chǎn)品名稱:Gene Pulser Xcell 電穿孔系統(tǒng) bio-rad伯樂(lè)/bio-rad轉(zhuǎn)基因系統(tǒng)
Gene Pulser Xcell 電穿孔系統(tǒng)
【介紹】
電穿孔是功能強(qiáng)大的將核酸、蛋白及其它分子導(dǎo)入多種細(xì)胞的高效技術(shù)。通過(guò)高強(qiáng)度的電場(chǎng)作用,瞬時(shí)提高細(xì) 胞膜的通透性,從而吸收周圍介質(zhì)中的外源分子。這種技術(shù)可以將核苷酸、DNA 與RNA、蛋白、糖類、染料及病 毒顆粒等導(dǎo)入原核和真核細(xì)胞內(nèi)。電轉(zhuǎn)化相對(duì)其它物理和化學(xué)轉(zhuǎn)化方法,是一種有價(jià)值和有效的替代方法。
Gene Pulser Xcell 系統(tǒng)的設(shè)計(jì),基于Bio-Rad 15 年來(lái)在電轉(zhuǎn)化技術(shù)上經(jīng)驗(yàn)積累,它提供指數(shù)波和方波波型選擇、 系統(tǒng)配置選擇及友好的用戶界面。
主要特點(diǎn)

指數(shù)波和方波波型確保所有細(xì)胞類型(原核及真核)均可獲得*佳 的電轉(zhuǎn)化效果

Bio-Rad **的
* PulseTrac 電路和電弧保護(hù)設(shè)計(jì),確??芍貜?fù)性并保護(hù)樣品

模塊化設(shè)計(jì)可根據(jù)研究需要選擇系統(tǒng)

用戶友好的數(shù)字化界面,具有直觀的編程以控制所有參數(shù), 包括附屬模塊的參數(shù)

包括人工操作、預(yù)設(shè)規(guī)程、用戶規(guī)程、一個(gè)優(yōu)化規(guī)程及其它先進(jìn) 功能等程序選擇
* 美國(guó)**4,750,100 和4,910,140。
指數(shù)波或方波脈沖選擇
Gene Pulser Xcell 系統(tǒng)可產(chǎn)生指數(shù)波和方波波型,使你選擇*適合你細(xì)胞的波型與規(guī)程。指數(shù)波和方波均能有效地用于電轉(zhuǎn)化及 電融合。電穿孔波型對(duì)不同類型細(xì)胞的轉(zhuǎn)化效率有很重要的影響。
PulseTrac 電路和電弧保護(hù)提供可靠的、可重復(fù)的和**的性能
Bio-Rad **的**微處理器控制電路能為任何規(guī)程提供可重復(fù)的結(jié)果,無(wú)論使用何種介質(zhì),它所產(chǎn)生的電壓值可在全世界范圍內(nèi)測(cè)試。PulseTrac 電路和電弧保護(hù):

確保電壓準(zhǔn)確地傳送到電擊杯

把CE 模塊中的低壓電容器**度從20% 提升到10%

便于電容器再校正,以保持**的脈沖指標(biāo),修正電容器隨著時(shí)間而發(fā)生的偏移

提供脈沖前樣品電阻測(cè)量

當(dāng)脈沖或電路中斷時(shí),可**地自動(dòng)放電

降低電弧危險(xiǎn),保護(hù)設(shè)備及樣品
用戶友好界面,便于編程和控制
主單元上的圖形界面可控制包括任何連接的附屬模塊在內(nèi)的所有功能。界面由一個(gè)通過(guò)功能鍵和字母數(shù)字鍵盤 操作的屏幕組成。根據(jù)屏幕提示進(jìn)行簡(jiǎn)便而直觀的編程。屏幕用于編程和顯示儲(chǔ)存與設(shè)定的程序、運(yùn)行參數(shù)以及波型。規(guī)程包括:

人工編程以輸入或編輯方波或指數(shù)衰變脈沖的所有參數(shù)

輔助編程需要的時(shí)間常數(shù)

預(yù)設(shè)有適合常用細(xì)菌、真菌和哺乳動(dòng)物細(xì)胞株的優(yōu)化程序

優(yōu)化規(guī)程

根據(jù)不同的波型選擇,顯示時(shí)間常數(shù)、實(shí)際電壓、脈沖間隔和脈沖時(shí)間等參數(shù)

儲(chǔ)存和調(diào)用前100 個(gè)實(shí)驗(yàn)的脈沖參數(shù)
預(yù)優(yōu)化規(guī)程,可快速選擇程序
這項(xiàng)功能為每個(gè)細(xì)胞株提供一組專用的預(yù)優(yōu)化參數(shù)。
提供以下細(xì)胞株的預(yù)設(shè)程序
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哺乳動(dòng)物
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細(xì)菌
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真菌
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CHO
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Escherichia coli
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Saccharomyces cerevisiae
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COS-7
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Agrobacterium tumefaciens
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Pichia pastoris
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3T3
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Pseudomonas aeruginosa
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Candida albicans
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293
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Staphylococcus aureus
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Schizosaccharomyces pombe
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HeLa
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Bacillus cereus
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Dictyostelium discoideum
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BHK21
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Streptococcus pyrogenes
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A549
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Lactobacillus plantarum
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CV1
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K562
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HL60
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Jurkat
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HuT78
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這些細(xì)胞株的詳細(xì)參數(shù)及制備電感受態(tài)細(xì)胞的詳細(xì)信息可查詢Gene Pulser Xcell 系統(tǒng)指導(dǎo)手冊(cè)或 discover.bio-rad.com 網(wǎng)站。
選擇你所需要的系統(tǒng)
Gene Pulser Xcell 是由一個(gè)主單元、2 個(gè)附屬模塊(CE 與PC 模塊)及ShockPod 電擊槽組成的模塊式電穿 孔系統(tǒng)。CE 模塊與Gene Pulser Xcell 主單元配合使用可電轉(zhuǎn)化多數(shù)真核細(xì)胞(包括哺乳動(dòng)物細(xì)胞和植物原生質(zhì) 體)。PC 模塊建議用于細(xì)菌和真菌的電轉(zhuǎn)化,或其它需要在小體積和高電阻樣品上施加高電壓脈沖的應(yīng)用。系統(tǒng)有 3 種組合。
Gene Pulser Xcell 選擇系統(tǒng)
【技術(shù)指標(biāo)】
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Gene Pulser Xcell 完全系統(tǒng)包括主單元、CE模塊、PC模塊
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輸出
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波型:指數(shù)衰變或方波
電壓: 10–3,000 V
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電容
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10–500 V, 25–3,275 μF , 25 μF 增量
500–3,000 V, 10, 25, 50 μF
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電阻(并聯(lián))
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50–1,000 ? ,50 ? 增量,加無(wú)窮大
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樣品電阻
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10–2,500 V 內(nèi),*小20 ?
2,500–3,000 V 內(nèi),*小600 ?
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方波時(shí)間
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10–500 V: 持續(xù)時(shí)間0.05–10 ms ,0.05 ms 增量;
持續(xù)時(shí)間10–100 ms ,1 ms 增量,1–10 個(gè)脈沖,間隔0.1–10 s
500–3,000 V:
持續(xù)時(shí)間0.05-5 ms,0.05 ms 增量,1-2 個(gè)脈沖, *小間隔5s
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Gene Pulser Xcell 真核系統(tǒng)包括主單元、CE 模塊;
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輸出和其他規(guī)格
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除了無(wú)并聯(lián)電阻外,輸出等與完全系統(tǒng)相同
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Gene Pulser Xcell 微生物系統(tǒng)
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包括主元件、PC 模塊
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輸出
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波型:指數(shù)衰變或方波
電壓:200–3,000 V
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電容
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10, 25, 50 μF
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電阻(并聯(lián))
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50–1,000 ? 增量50 ? ,加無(wú)窮大
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樣品電阻
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200–2,500 V 內(nèi),*小20 ?
2,500–3,000 V 內(nèi),*小600 ?
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方波時(shí)間
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持續(xù)時(shí)間0.05–5 ms , 0.05 ms 增量,1-2 個(gè)脈沖,*小間隔5s
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Gene Pulser Xcell 主單元
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輸出
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波型:指數(shù)衰變或方波
電壓:200–3,000 V
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放電電容
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10, 25, 50 μF
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樣品電阻
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200–2,500 V 內(nèi),*小20 ?
2,500–3,000 V 內(nèi),*小600 ?
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方波時(shí)間
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持續(xù)時(shí)間0.05–5 ms , 0.05 ms 增量,1-2 個(gè)脈沖,*小間隔5s
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常規(guī)
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輸入電壓
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100–120 VAC 或220–240 VAC, 50/60 Hz
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電源
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*大240 W (在短放電過(guò)程中)
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工作環(huán)境
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溫度0–35°C, 濕度0–95% (無(wú)冷凝水)
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**認(rèn)證
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EN61010, EMC EN61326 A 級(jí)**認(rèn)證
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大小(W x D x H)
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主單元:31 x 30 x 14 cm
CE 模塊: 31 x 28 x 9 cm
PC 模塊: 31 x 28 x 5 cm
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重量
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主單元: 6.6 公斤
CE 模塊: 3.1 公斤
PC 模塊:1.9 公斤
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【訂貨信息】
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目錄 #
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描述
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Systems
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165-2660
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Gene Pulser Xcell Total System, for mammalian and microbial cells, 100/240 V, 50/60 Hz, exponential and square-wave delivery, includes main unit, CE module, PC module, ShockPod chamber, 15 sterile cuvettes (5 each of 0.1, 0.2, and 0.4 cm gap), instructions
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165-2661
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Gene Pulser Xcell Eukaryotic System, 100/240 V, 50/60 Hz, exponential (25–3,275 μF range) and square-wave delivery, includes main unit, CE module, ShockPod chamber, 5 sterile cuvettes (0.4 cm gap), cuvette rack, instructions
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165-2662
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Gene Pulser Xcell Microbial System, 100/240 V, 50/60 Hz, exponential decay delivery, includes main unit, PC module, ShockPod chamber, 10 sterile cuvettes (5 each of 0.1 and 0.2 cm gap), cuvette rack, instructions
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產(chǎn)品名稱:電擊杯/電轉(zhuǎn)杯 bio-rad伯樂(lè)/bio-rad轉(zhuǎn)基因系統(tǒng)
電 擊 杯
【介紹】
Bio-Rad 高品質(zhì)的電擊杯為您寶貴的樣品提供穩(wěn)定的脈沖傳送,確保結(jié)果的重復(fù)性。電擊杯有3 種不同的電極間距 — 0.4、0.2 和0.1cm,針對(duì)不同的細(xì)胞類型選擇*理想的場(chǎng)強(qiáng)。電擊杯的特點(diǎn)包括:

保證效率— 使用這些電擊杯確保*高電轉(zhuǎn)化效率,制作精良的電擊間距公差保證了實(shí)驗(yàn)間的重復(fù)性

確保無(wú)菌— 每一個(gè)電擊杯都在凈室環(huán)境下裝配、洗滌、加蓋和包裝,并經(jīng)過(guò)gamma 射線滅菌

結(jié)構(gòu)堅(jiān)固— 耐用的聚碳酸酯能承受極高的電壓

標(biāo)有顏色的蓋和包裝袋— 可快速地區(qū)分不同規(guī)格的電擊杯

一致的腔體形狀— 無(wú)縫塑料模制避免了滲漏并保證鋁板平行— 這是均一的樣品處理和**性的關(guān)鍵所在

平滑的電極表面— 鋁片經(jīng)過(guò)11步嚴(yán)格的蝕刻和清洗處理,確保對(duì)整個(gè)樣品的一致性脈沖傳送
電擊杯有3 種包裝規(guī)格。標(biāo)準(zhǔn)包裝含50個(gè)單獨(dú)包裝的無(wú)菌電擊杯;大包裝含500 個(gè)無(wú)菌電擊杯,經(jīng)濟(jì)并且減少了不必要的包裝。小包裝含 5 個(gè)無(wú)菌電擊杯,是只需要少許電擊杯實(shí)驗(yàn)室的理想選擇。
電擊杯選擇指南
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0.4 cm 間距電擊杯
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寬間距、低場(chǎng)強(qiáng)、應(yīng)用于哺乳動(dòng)物細(xì)胞和其它真核細(xì)胞
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0.2 cm 間距電擊杯
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窄間距、高場(chǎng)強(qiáng)、應(yīng)用于酵母、細(xì)菌和其它真核細(xì)胞
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0.1 cm 間距電擊杯
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*窄間距、極高的場(chǎng)強(qiáng),淺底用于小體積樣品(40–80 μl)應(yīng)用于酵母和細(xì)菌轉(zhuǎn)化
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【訂貨信息】
|
目錄 #
|
描述
|
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165-2081
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Gene Pulser/MicroPulser Cuvettes, 0.4 cm gap, 5 (mini pack)
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165-2082
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Gene Pulser/MicroPulser Cuvettes, 0.2 cm gap, 5 (mini pack)
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165-2083
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Gene Pulser/MicroPulser Cuvettes, 0.1 cm gap, 5 (mini pack)
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165-2086
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Gene Pulser/MicroPulser Cuvettes, 0.2 cm gap, 50 (regular pack)
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165-2088
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Gene Pulser/MicroPulser Cuvettes, 0.4 cm gap, 50 (regular pack)
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165-2089
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Gene Pulser/MicroPulser Cuvettes, 0.1 cm gap, 50 (regular pack)
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165-2091
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Gene Pulser/MicroPulser Cuvettes, 0.4 cm gap, 500 (jumbo pack)
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165-2092
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Gene Pulser/MicroPulser Cuvettes, 0.2 cm gap, 500 (jumbo pack)
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165-2093
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Gene Pulser/MicroPulser Cuvettes, 0.1 cm gap, 500 (jumbo pack)
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產(chǎn)品名稱:MicroPulser 電穿孔儀 bio-rad伯樂(lè)/bio-rad轉(zhuǎn)基因系統(tǒng)
MicroPulser 電穿孔儀
【介紹】
MicroPulser 電穿孔儀是一種簡(jiǎn)單而靈活的儀器,能**而可重復(fù)地轉(zhuǎn)化細(xì)菌、酵母菌和其它微生物體。轉(zhuǎn)化效率大大高于化學(xué)方法。系統(tǒng)的獨(dú)特性包括:

簡(jiǎn)單的單鍵式脈沖激發(fā)、附著的電擊杯槽、快速充電等特點(diǎn)便于更快速的樣品操作

方便的預(yù)設(shè)優(yōu)化程序適合常用的細(xì)菌和真菌研究

電弧抑制系統(tǒng)大大減少了電弧,確保珍貴樣品不受損失

可人工調(diào)節(jié)多項(xiàng)參數(shù);電壓可在200-3000V 間選擇,精度10V;時(shí)間常數(shù)可在1.0-4.0 ms 范圍內(nèi)調(diào)節(jié),精度0.1 ms

可提供3000V 電壓,提高更大容積比色杯的轉(zhuǎn)化效率

設(shè)計(jì)小巧、節(jié)約空間

有聲且可視的脈沖顯示器

顯示時(shí)間常數(shù)和實(shí)際電壓,監(jiān)控其可重復(fù)性
【技術(shù)指標(biāo)】
|
輸入電壓
|
100–120 V 或220–240 V
|
|
*大輸出電壓和電流
|
3,000 V 峰值, >600 W 負(fù)載。峰值*大電流100 A
|
|
輸出波型
|
帶RC 時(shí)間常數(shù)的衰變或斜截衰變指數(shù)波型
|
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輸出電壓和脈沖持續(xù)調(diào)節(jié)
|
電壓在200-3,000V 范圍內(nèi)調(diào)節(jié),**度10 V;
缺省值5 ms,或1-4 ms 精度0.1 ms;5 個(gè)細(xì)菌和5 個(gè)真菌預(yù)設(shè)程序
|
|
工作溫度
|
3.5–35oC
|
|
大小(W x D x H)
|
29 x 21 x 8 cm
|
|
重量
|
2.9 kg
|
【訂貨信息】
|
目錄 #
|
描述
|
|
165-2100
|
MicroPulser Electroporator, includes a cuvette chamber with leads, 10 sterile cuvettes (5 each of 0.1 cm and 0.2 cm gap)
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