5D-82411(S,A,B) IFU V2 Revision Date 20191031
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5D PROTEIN STABILIZER
(10% Hydrolyzed Porcine
Collagen Peptides)
5D-82411S
50 mL
5D-82411A
100 mL
5D-82411B
1000 mL
For Laboratory Use Only.
Not for use in human or direct animal applications.
Store at 2-8°C
5D Protein Stabilizer stabilizes and increases the shelf life of
proteins and enzymes and is a BSA alternative with minimal
lot to lot variation.
DESCRIPTION
Hydrolyzed porcine collagen peptide fraction, from dermal
origin, which can be used as a protein stabilizer for
lyophilized or liquid formulations, or as a blocking agent for
coated surfaces. 5D Protein Stabilizer is free from cartilage,
bone and plasma components. The product is sterilized for a
long-term storage.
FORMULA
Pig collagen peptides, from hydrolyzed collagen, heat stable.
MOLECULAR WEIGHT
10 to 20 kDa
COMPOSITION
Aqueous solution containing 10% hydrolyzed collagen
peptides, free of any additives. pH of around 7.00.
SOLUBILITY
Totally soluble in water.
PORCINE COLLAGEN PEPTIDES ACTIVITY
Unknown toxic and non-antigenic activity.
PRINCIPLE
Concentrated solution of hydrolyzed porcine collagen
peptides, highly efficient as protein stabilizer.
PREPARATION
Obtained from purified porcine collagen, diluted at a 10%
(100 mg/ml) concentration, hydrolyzed at high temperature,
filtrated on a 0.2 μ filter and sterilized.
APPLICATIONS
Can be used for protein stabilization, in lyophilized
formulations or in liquid solutions, during urea, ethanol or
heat treatment. Applications as active sites blocker in ELISAs
or on coated surfaces.
STORAGE AND STABILITY
Stable until the expiration date printed on the bottle label,
when stored at 2-8°C.
Can be frozen.
As 5D Protein Stabilizer contains no preservatives, when
open the bottle must be used within 24 hours and any
contamination must be avoided.
Alternatively, 5D Protein Stabilizer when open can be
supplemented with preservatives for preventing
microbiological or fungal contamination and growth (sodium
azide at 0.9 mg/ml final or BNP at 0.1 mg/ml are
recommended).
Protect from light.
WARNINGS AND PRECAUTIONS
For Laboratory Use Only.
Not for use in human or direct animal applications.
REFERENCES
1. Gaffney PJ, Edgell TA, Dawson PA, Ford AW, Stocker E, A
pig collagen peptide fraction. A unique material for
maintaining biological activity during lyophilization and
during storage in the liquid state, J Pharm Pharmacol.
1996 Sep;48(9):896-8
2. Armin Baur, Ralf Reski, Gilbert Gorr, Enhanced recovery
of a secreted recombinant human growth factor using
stabilizing additives and by co-expression of human
serum albumin in the moss Physcomitrella patens, Plant
Biotechnology Journal (2005) 3, pp. 331–340
3. C. Berrettoni, C. Trono, S. Berneschi, A. Giannetti, S.
Tombelli, R. Bernini, A. Grimaldi, G. Persichetti, G. Testa,
L. Bolzoni, G. Porro, H.Becker, C. G?rtner, F. Baldini, A
newly designed optical biochip for a TDM – POCT device,
Proceedings of SPIE - The International Society for
Optical Engineering, (2014); Vol. 8976
4. Kerstin von Kolontaj, Gabor L. Horvath, Eicke Latz, Martin
Büscher, Automated Nanoscale Flow Cytometry for
Assessing Protein–Protein Interactions, Cytometry Part A
_ 89A: 835_843, (2016)
Heuberg 7, 4051 Basel,
Switzerland
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