Package Contents:
500 ugs – 1 vial of 500 ugs lyophilized SOL-CTZ
1 mgs – 2 vials of 500 ugs lyophilized SOL-CTZ
10 mgs – 20 vials of 500 ugs lyophilized SOL-CTZ
Coelenterazine is often dissolved in Propyleneglycol (PG) in combination with other alcohols for in vivo imaging studies. Large intravenous doses of PG given over a short period of time can be toxic, including hyperosmolality, increased anion gap metabolic acidosis (due to lactic acidosis), acute kidney injury, and sepsis-like syndrome.
This can be avoided using water-soluble Coelenterazine. It is formulated to be isosmotic and easily secreted by the kidneys. Thus save for repeated i.v. injections. Water soluble Coelenterazine can be injected up to a 500 μg/100 μl concentration, achieving very high Coelenterazine levels within the body and leading to high Gaussia luciferase signals.
Application Example
Controls
We offer the solubilization vehicle without the Coelenterazine as injection control. Any side effects due to the injection process can be easily monitored, especially in sensitive applications like bioluminescent driven Optogenetics. One vial of the control (Cat. #3031-10+C) contains the same amount of solubilizer as one vial of Cat.#3031. The control will be dissolved in sterile water to create an iso-osmotic solution ready to inject.
To order control: please select ’10 mgs + 10 vials control’ under product options. This includes 20 vials of 500ug water-soluble CTZ and 10 vials of control.
Please inquire if larger quantities of Cat.#3031-10+C are needed.
