產(chǎn)品介紹
Rabbit Anti-Borrelia burgdorferi sensu stricto (B31) P39 Antibody
PAB21457-25 、PAB21457-100
25 μl (1.0 mg/mL by UV absorbance at 280 nm)、0.1 ml (1.0 mg/mL by UV absorbance at 280 nm)
Polyclonal
Rabbit IgG
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
0.01% (w/v) Sodium Azide
Recombinant MBP tagged B. burgdorferi P39 protein
Protein-A purified and cross-adsorbed against MBP from monospecific antiserum by chromatography
This antibody is specific for Borrelia burgdorferi P39 protein. A BLAST analysis was used to suggest cross-reactivity with p39 from B. burgdorferi and B. afzelii sources based on 100% homology with the immunizing sequence. Partial reactivity is expected against B. garinii sources based on 88-95% homology and to B. spielmanii A14S based on 86-96% homology. Crossreactivity with p39 from other sources has not been determined.
ELISA (1:5000), WB (1:1000)
Store vial at -20° C or below prior to opening. To minimize loss of volume, dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended above. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
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