最近最新的日本中文MV_麻豆出品视频在线_超pen个人视频97_国产精品第一国产精品_国产香蕉片_免费看麻豆文化传媒_奶大灬舒服灬太大了一进一出_人妻激情偷乱一区二区三区

This antiserum was produced against canine cardiac sarcolemmal Na+ -Ca2+ exchanger (NCX1; full length isolated protein). The specific epitope was never mapped, but is likely to be primarily on the large intracellular loop. Brain, smooth muscle and kidney Na+ -Ca2+ exchanger are also recognized by the antiserum. The antiserum cross reacts with the proteins of mouse, rat, rabbit, frog, toad, guinea pig, cow and human. It does not react with NCX2 or NCX3. The antiserum can be used for immunoblots, immunoprecipitation and immunohistochemistry.  

 Immunohistochemistry; 1:5’000 - 1: 10’000 with the avidin-biotin method 

Immunoblots: 1:100 – 1: 1’000 (use milk for blocking). Primary bands at 120 kDa (full length protein), 160 kDA (some non-reduced protein even in the presence of DDT) and 70 kDa (active proteolytic fragment). 

We recommend that the optimal dilutions be determined by titration experiments. 

 For long storage, keep at - 80°C (or at least - 20°C). For continuous use keep at 4°C (with 0.01% Na-azide). Avoid repeated freezing and thawing.

 Philipson K.D. et al. (1988) Purification of the cardia Na+ -Ca2+ exchanger protein. Biochim. Biophys. Acta 945: 298-306.