產(chǎn)品介紹
Monoclonal anti parvalbumin
235
200 μl、500 μl 、1 ml
monoclonal
nonconjugated
zebra finch, human, mouse, rat, zebrafish , domestic sheep, rhesus macaque
immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section, immunohistochemistry - free floating section
Mouse monoclonal, IgG1
Conc. supernat. lyophil.
Lyophilized ascites (no preservatives).
with 200 μl bidistilled water.
Monoclonal anti-Parvalbumin is a mouse IgG1 produced by hybridization of mouse myeloma cells with spleen cells from mice immunized with parvalbumin purified from carp muscles (1). The antibody was evaluated for specificity and potency: a) by indirect immunofluorescent or immunoperoxidase labeling as well as Avidin-Biotin staining of cryostat or vibratome-sections of 4% paraformaldehyde fixed tissue; b) by immunoenzymatic labelling of immunoblots; c) by radioimmunoassay (RIA)(1). The product is a monoclonal antibody (McAB) against parvalbumin, a calcium-binding protein of the EF-hand family related to calmodulin and troponin-C. The antibody reacts specifically with parvalbumin in tissue originating from human, monkey, rabbit, rat, mouse chicken and fish. The McAB specifically stains the 45Ca-binding spot of parvalbumin (MW 12'000 and IEF 4.9) in a two-dimensional "immunoblot". In a RIA set up the McAB measures parvalbumin with a sensitivity of 10 ng/assay and an affinity of 7.9 x 1012 L/M (1).
Immunohistochemistry: 1:5'000 - 1:10'000
Immunoblotting: 1:1'000.
We recommend that the optimal dilution be determined by titration test.
For long storage, keep small aliquots of 1-2 μl at - 80°C (or at least - 20°C). For continous use keep diluted solutions at 4°C (with 0.01% Na-azide). Avoid repeated freezing and thawing
1. Celio M.R. et al., Cell Calcium 9:81-86, 1988
2. Celio M.R., Heizmann C.W. (1981) Nature 293: 300-302
3. Celio M.R., Heizmann C.W. (1982) Nature 297:504-506
4. Celio M.R. (1986) Science 231:995-997
5. Schwaller B., et al. (1999) Am. J. Physiol. 276. C395-403
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