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western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section, immunohistochemistry - free floating section

Goat

Lyophilized antiserum.

 with 200 μl bidistilled water.

This antibody was produced against human recombinant calretinin. It can be used in immunohistochemistry and in immunoblots.

Immunohistochemistry: 1:1'000 - 1:10'000 with the avidin-biotin method. 

Immunoblots: 1:1'000. 

We recommend that the optimal dilutions be determined by titration experiments.

For long storage, keep at - 80°C (or at least - 20°C). For continuous use keep at 4°C (with 0.01% Na-azide). Avoid repeated freezing and thawing.

1. Schiffmann S.N. et al (1999) Impaired motor coordination and Purkinje cell excitability in mice lacking calretinin. PNAS, 96: 5257-5262.