產(chǎn)品介紹
Rabbit anti-calretinin
7697
200 μl
polyclonal
nonconjugated
human, mouse, leopard danio
immunohistochemistry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section
Rabbit
Antiserum lyophil.
Lyophilized whole serum (no preservatives).
with 200 μl of bidistilled water
The antiserum against calretinin is produced in rabbits by immunization with recombinant human calretinin containing a 6-his tag at the N-terminal. The antibody was evaluated for specificity and potency: a) by Biotin-Avidin labeling of cryostate-, vibratome- and paraffinsections of 4% paraformaldehyde fixed brains and b) by immunoenzymatic labelling of immunoblots。
Immunohistochemistry: 1:2'000 - 1:5'000, on paraformaldehyde (4%) or formalin-fixed tissue, cryostate or paraffin-sections.
Immunoblots: 1:1'000 - 1:2'000.
For immunohistochemistry and immunoblots the titer was determined by using the avidin-biotin method. We recommend that the optimal dilutions be determined by titration experiments.
After reconstitution freeze in small aliquots (e.g. 1 μl) and keep at - 80°C (or at least - 20°C). For continuous use, keep at 4°C (with 0.01% Na-azide). Avoid repeated freezing and thawing.
1. Schwaller B., Buchwald P., Blümcke I., Celio M.R. and Hunziker W. (1994) Characterization of a polyclonal antiserum against the purified human recombinant calcium-binding protein calretinin. Cell Calcium 14: 639-648.
2. Schiffmann S.N. et al (1999) Impaired motor coordination and Purkinje cell excitability in mice lacking calretinin. PNAS, 96: 5257-5262.
3. Gotzos V., Vogt P. and M.R. Celio (1995) Calretinin is a selective marker for malignant pleural mesotheliomas of the epithelial type. Pathol. Res. Pract. 192:137- 147.
4. Doglioni, C. et al. (1996) Calretinin: a novel immunocytochemical marker for mesothelioma. Am. J. Surg. Pathol. 20:1037-1046.
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