產(chǎn)品介紹
Affinity purified mouse anti-calretinin (monoclonal)
6B3PUR
100ul
Purified antibody in PBS (lyophilized).
with 100 μl bi-distilled water
The antibody 6B3pur was purified with Protein-G from in vitro produced monoclonal antibody 6B3 directed against calretinin (see product description 6B3 for further details concerning immunoblot and immunohistochemistry). The antibody concentration is of 0.29 mg/ml in 0.1M phosphate buffered saline, pH 7.3.
Immunohistochemistry: we suggest using a dilution of 1:10’000 (0.029 ?g/ml) for immunostaining with the avidin-biotin method on cryostat or paraffin-sections of paraformaldehyde (4%) or formalin-fixed tissue.
We recommend that the optimal dilutions be determined by titration experiments
After reconstitution freeze in small aliquots (e.g. 1 μl) and keep at - 80°C (or at least - 20°C). For continuous use, keep at 4°C (with 0.01% Na-azide). Avoid repeated freezing and thawing.
1. Zimmermann, L. and Schwaller B (2002) Monoclonal antibois recognizes epitopes of calretinin. Cell Calcium 31: 13-25.
2. Rogers J.H. (1987) Calretinin: a gene for a novel calcium-binding protein expressed principally in neurons. J. Cell Biol. 105:1343-1353.
3. Gotzos V., Vogt P. and M.R. Celio (1995) Calretinin is a selective marker for malignant pleural mesotheliomas of the epithelial type. Pathol. Res. Pract. 192:137-147.
4. Doglioni, C. et al. (1996) Calretinin: a novel immunocytochemical marker for mesothelioma. Am. J. Surg. Pathol. 20:1037-1046.
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