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Baboon, Horse, Hamster, Rabbit, Guinea Pig, Rat, Mouse, Rhesus macaque

2°C to 8°C  (≤ 30 days)

For long-term storage (> 30 days), aliquot and store at ≤ –20°C. Avoid repeated freeze-thaw.

For Research Use Only. Not for use in diagnostic procedures

Quidel Polyclonal Antiserum to Human C3 Protein

Highly purified human C3 was isolated from normal serum and used to immunize goats. The Anti-Human C3 Polyclonal Antisera was tested against normal human plasma by double immunodiffusion, one-dimensional immunoelectrophoresis, quantitative radial immunodifussion, and quantitative rocket immunoelectrophoresis. The antiserum was determined to be monospecific for C3 at varying concentrations.

C3 plays a central role for the classical, alternative, and lectin pathways of the complement system. The circulating form of C3 is naturally glycosylated and contains two disulfide-bonded chains that weigh approximately 110 kD and 75 kD, respectively. The average concentration of circulating C3 in human serum/plasma is 1.25 mg/mL. 

Activation of any of the three complement pathways results in the cleavage of C3 into C3a and C3b fragments. C3a is one of the three complement-derived anaphylatoxins. The C3a polypeptide also exhibits immunoregulatory activity. This activity is dependent on the physical binding of C3a to helper T lymphocytes, which results in the inhibition of helper T cell function.

Applications of the C3 polyclonal antisera have been evaluated by various research facilities, and include Western Blot, 1 Flow Cytometry, 2 IHC, 3 Immunoblot, 4 and ELISA. 5