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DiscoverX Wee1, active 14-925

品牌：Eurofins DiscoverX

貨號(hào)：14-925

規(guī)格：14-925

貨期：DiscoverX

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品牌：DiscoverX 品名：Wee1, active 貨號(hào)：14-925 規(guī)格：10 μg

商品詳情參考文獻(xiàn) 相關(guān)資料

品牌：DiscoverX
品名：Wee1, active
貨號(hào)：14-925
規(guī)格：10 μg

簡(jiǎn)要說明：

Wee1, active

N-terminal GST-tagged and C-terminal 6His-tagged recombinant, human Wee1 amino acids 214-end. Acts as a negative regulator of entry into mitosis (G2 to M transition) by protecting the nucleus from cytoplasmically activated cyclin B1-complexed CDK1 before the onset of mitosis by mediating phosphorylation of CDK1 on 'Tyr-15'. Specifically phosphorylates and inactivates cyclin B1-complexed CDK1 reaching a maximum during G2 phase and a minimum as cells enter M phase. Phosphorylation of cyclin B1-CDK1 occurs exclusively on 'Tyr-15' and phosphorylation of monomeric CDK1 does not occur. Its activity increases during S and G2 phases and decreases at M phase when it is hyperphosphorylated. A correlated decrease in protein level occurs at M/G1 phase, probably due to its degradation.

產(chǎn)品介紹：

Target

WEE1

Expression System

Expressed by baculovirus in Sf21 insect cells

Application

Kinase Assay

Molecular Weight

77 kDa

Accession Number

NM_003390

Species

Human

Tag

GST, 6His

Purification Method

Glutathione agarose affinity chromatography

Content

Protein in 50mM Tris/HCl pH7.5, 300mM NaCl, 0.1mM EGTA, 0.03% Brij-35, 270mM sucrose, 1mM benzamidine, 0.2mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution.

Storage

6 months at -70°C

Bulk Sizes

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