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DiscoverX ULK2, active 14-772M

品牌：Eurofins DiscoverX

貨號：14-772M

規(guī)格：14-772M

貨期：DiscoverX

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品牌：DiscoverX 品名：ULK2, active 貨號：14-772M 規(guī)格：250 μg

商品詳情參考文獻(xiàn) 相關(guān)資料

品牌：DiscoverX
品名：ULK2, active
貨號：14-772M
規(guī)格：250 μg

簡要說明：

ULK2, active

N-terminal GST and C-terminal 6His-tagged recombinant, human, ULK2 amino acids 1–306. Serine/threonine-protein kinase involved in autophagy in response to starvation. Acts upstream of phosphatidylinositol 3-kinase PIK3C3 to regulate the formation of autophagophores, the precursors of autophagosomes. Part of regulatory feedback loops in autophagy: acts both as a downstream effector and a negative regulator of mammalian target of rapamycin complex 1 (mTORC1) via interaction with RPTOR. Activated via phosphorylation by AMPK, also acts as a negative regulator of AMPK through phosphorylation of the AMPK subunits PRKAA1, PRKAB2 and PRKAG1. May phosphorylate ATG13/KIAA0652, FRS2, FRS3 and RPTOR; however such data need additional evidences. Not involved in ammonia-induced autophagy or in autophagic response of cerebellar granule neurons (CGN) to low potassium concentration. Plays a role early in neuronal differentiation and is required for granule cell axon formation: may govern axon formation via Ras-like GTPase signaling and through regulation of the Rab5-mediated endocytic pathways within developing axons.

產(chǎn)品介紹：

Target

ULK2

Expression System

Expressed by baculovirus in Sf21 insect cells

Application

Kinase Assay

Molecular Weight

62 kDa

Accession Number

NM_014683

Species

Human

Tag

GST, 6His

Purification Method

Glutathione agarose affinity chromatography

Content

Protein in 50mM Tris/HCl pH7.5, 300mM NaCl, 0.1mM EGTA, 0.03% Brij-35, 270mM sucrose, 1mM benzamidine, 0.2mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution.

Storage

6 months at -70°C

Bulk Sizes

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