Catalogue number
PRO-1962
Synonyms
ATPase Na+/K+ Transporting Beta 3 Polypeptide, Sodium-Potassium ATPase Subunit Beta 3 (Non-Catalytic), Sodium/Potassium-Transporting ATPase Subunit Beta-3, Sodium/Potassium-Dependent ATPase Subunit Beta-3, Sodium Pump Subunit Beta-3, ATPB-3, Sodium/Potassium-Transporting ATPase Beta-3 Chain, Sodium/Potassium-Dependent ATPase Beta-3 Subunit, Na K-ATPase Beta-3 Polypeptide, CD298 Antigen, CD298, ATP1B3.
Introduction
ATPaseTransporting Beta 3 (ATP1B3) is a member of the X(+)/potassium ATPases subunit beta family. ATP1B3 is the non-catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of Na+ and K+ ions across the plasma membrane. The beta subunit controls, by way of assembly of alpha/beta heterodimers, the quantity of sodium pumps transported to the plasma membrane. The precise role of the beta-3 subunit is unknown.
Description
ATP1B3 Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 246 amino acids (57-279) and having a molecular mass of 27.4 kDa.
ATP1B3 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Source
Escherichia Coli.
Physical Appearance
Sterile Filtered colorless solution.
Formulation
The ATP1B3 solution (0.25mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 20% glycerol, 0.15M NaCl and 1mM DTT.
Stability
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid multiple freeze-thaw cycles.
Purity
Greater than 80.0% as determined by SDS-PAGE.
Amino acid sequence
MGSSHHHHHH SSGLVPRGSH MGSTMWVMLQ TLNDEVPKYR DQIPSPGLMV FPKPVTALEY TFSRSDPTSY AGYIEDLKKF LKPYTLEEQK NLTVCPDGAL FEQKGPVYVA CQFPISLLQA CSGMNDPDFG YSQGNPCILV KMNRIIGLKP EGVPRIDCVS KNEDIPNVAV YPHNGMIDLK YFPYYGKKLH VGYLQPLVAV QVSFAPNNTG KEVTVECKID GSANLKSQDD RDKFLGRVMF KITARA.
Safety Data Sheet
Usage
ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
