Glycinin Ara h 3 is a seed storage protein, 11 S globulin and trypsin inhibitor from peanut. Each subunit of the hexamer is composed of an acidic and a basic chain derived from a single precursor and linked by a disulfide bond. Ara h 3 and Ara h 4 are isoforms. Glycinin is the source of sulfur-containing amino acids in seed meals and it exists in the seeds of many leguminous and non-leguminous plants.
Recombinant Glycinin produced in SF9 is a glycosylated, polypeptide chain having a calculated molecular mass of 60,069 Dalton.
Glycinin is expressed with a 10xHis tag at N-terminus and purified by proprietary chromatographic techniques.
Sf9 insect cells.
Sterile Filtered clear solution.
Glycinin is supplied in 20mM HEPES buffer pH-7.9 and 6M Urea.
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
Avoid multiple freeze-thaw cycles.
Greater than 80.0% as determined by SDS-PAGE.
1. Binds IgE type human antibodies.
2. Immunodot test with positive/negative sera panels.
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