Catalogue number
ANT-534
Synonyms
APG3,
APG3-LIKE, APG3L, PC3-96, Ubiquitin-like-conjugating enzyme ATG3,
Autophagy-related protein 3, hApg3, PC3-96, ATG3.
Type
Mouse Anti Human Monoclonal.
Introduction
Autophagy Related 3 (ATG3) is a conjugating enzyme essential for the cytoplasm to vacuole transport (Cvt), autophagy, and mitochondrial homeostasis. ATG3 is localizes to the cytoplasm and is expressed in tissues with predominant levels found in kidney, placenta, liver, heart and skeletal muscle. ATG3 catalyzes a reaction which is necessary for autophagy. That reaction is the formation of the Atg8-phosphatidylethanolamine (ATG-PE) conjugate.
Clone
Physical Appearance
Sterile filtered colorless solution.
Immunogen
Anti-human ATG3mAb, clone PAT15F6AT, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human ATG3 protein (1-314 amino acids purified from E. coli.
Ig Subclass
Purification Method
ATG3 antibody was purified from mouse ascitic fluids by
protein-A affinity chromatography.
Formulation
1mg/ml containing PBS, pH-7.4, 10% Glycerol and 0.02% Sodium Azide.
Storage Procedures
For periods up to 1 month store at 4°C, for longer periods of time, store at -20°C.?
Prevent freeze thaw cycles.
Stability / Shelf Life
12 months at -20°C. 1 month at 4°C.
Applications
The antibody has been tested by ELISA, Western blot
analysis to assure specificity and reactivity. Since application varies,
however, each investigation should be titrated by the reagent to obtain optimal
results. Recommended starting dilution is 1:1000.
Safety Data Sheet
Usage
ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
