Catalogue number
ANT-053
Synonyms
Platelet endothelial cell adhesion molecule, PECAM-1, EndoCAM, GPIIA', CD31 antigen, PECAM1, CD31.
Type
Mouse Anti Human Monoclonal.
Introduction
CD31 is a glycoprotein expressed on endothelial cells, platelets, macrophages and Kupffer cells, granulocytes, T / NK cells, lymphocytes, megakaryocytes, fibroblasts, osteoclasts, neutrophils. In humans, the gene encoding CD31 is found on chromosome 17.
CD31 is a cluster of differentiation molecule. It is also called PECAM-1 for platelet endothelial cell adhesion molecule. It plays a key role in removing aged neutrophils from the body. Both the neutrophil and the macrophage express CD31 on their membranes, and during the testing process, these CD31 molecules bind the two cells together. If the neutrophil is healthy, it will send a signal to the macrophage, and the CD31 molecules will detach.
CD31 is also expressed in certain tumors, including epithelioid hemangioendothelioma, epithelioid sarcoma-like hemangioendothelioma, other vascular tumors, histiocytic malignancies, and plasmacytomas. It is rarely found in some sarcomas and carcinomas.
Clone
hCD31
Immunogen
Enriched human platelets
Ig Subclass
mouse IgG1
Purification Method
Protein A
Formulation
1mg/ml in PBS (after reconstitution).
Shipping Conditions
Antibody is shipped lyophilized at ambient temperature.
Storage Procedures
In lyophilized form, for long periods, store at 4°C in a dry environment. After reconstitution,
if not intended for use within a month, aliquot and store at -20°C.
Solubility
Reconstitute with H20. Mix gently, wash the sides of the vial and wait 30-60 seconds before use.
Applications
Flow cytometry, immunohistochemistry. For staining, use 10μl/106 cells.
Available Conjugates
This antibody is also available conjugated to FITC and biotin. For flow cytometry use 5-10μl/106 cells.
Note
CD31 is expressed in high levels on endothelial cells and in lower levels on monocytes, granulocytes and platelets.
Safety Data Sheet
Usage
ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
