Catalogue number
ANT-210
Synonyms
HIV-1 gp120, HIV1gp120, HIV-I gp120, HIVIgp120.
Type
Polyclonal Rabbit Antibody.
Introduction
Human immunodeficiency virus (HIV) is a retrovirusthat can lead to a condition in which the immune systembegins to fail, leading to opportunistic infections. HIV primarily infects vital cells in the humanimmune systemsuch as helper T cells(specifically CD4+ T cells), macrophagesand dendritic cells. HIV infection leads to low levels of CD4+ T cells through three main mechanisms: firstly, direct viral killing of infected cells; secondly, increased rates of apoptosisin infected cells; and thirdly, killing of infected CD4+ T cells by CD8 cytotoxic lymphocytesthat recognize infected cells. When CD4+ T cell numbers decline below a critical level, cell-mediated immunityis lost, and the body becomes progressively more susceptible to opportunistic infections. HIV was classified as a member of the genus Lentivirus, part of the family of Retroviridae. Lentiviruses have many common morphologies and biological properties. Many species are infected by lentiviruses, which are characteristically responsible for long-duration illnesses with a long incubation period. Lentiviruses are transmitted as single-stranded, positive-sense, enveloped RNA viruses. Upon entry of the target cell, the viral RNA genomeis converted to double-stranded DNAby a virally encoded reverse transcriptasethat is present in the virus particle. This viral DNA is then integrated into the cellular DNA by a virally encoded integraseso that the genome can be transcribed. Once the virus has infected the cell, two pathways are possible: either the virus becomes latentand the infected cell continues to function, or the virus becomes active and replicates, and a large number of virus particles are liberated that can then infect other cells.
Description
Rabbit Polyclonal Antibody to gp120 (HIV-1) serum.
Immunogen
Recombinant HIV-1 gp120 produced in baculovirus expression system.
Physical Appearance
Sterile filtered pinkish powder.
Formulation
Polyclonal Antibody serum was lyophilized in vials.
Stability
Stable at -20°C. Avoid freeze/thaw cycles.
Applications
Binds to recombinant HIV-1 gp120 ELISA and Western Blot Optimal dilutions should be determined by the customer, based on titration. Suggested starting dilutions are listed below: Western Blot: 1-500 to 1-1000. ELISA: 1:500 to 1000.
Safety Data Sheet
Usage
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
