Catalogue number
PRO-2182
Synonyms
Annexin A1, Annexin I, Annexin-1, Calpactin II, Calpactin-2,
Chromobindin-9, Lipocortin I, Phospholipase
A2 inhibitory protein, p35.
Introduction
ANXA1 is part of the family of Ca(2+)-dependent phospholipid binding proteins which have a Mw between 35kDa-40kDa and are situated on the cytosolic face of the plasma membrane. ANXA1 protein has a Mw of 40kDa, with phospholipase A2 inhibitory activity to bind from two to four calcium ions with high affinity. Since phospholipase A2 is necessary for the biosynthesis of the potent mediators of inflammation, prostaglandins and leukotrienes, ANXA1 might have potential anti-inflammatory activity. ANXA1 promotes membrane fusion and iplays a role in exocytosis. The recognition of ANXA1 protein by immunocytochemical leads a simple, highly sensitive and specific assay for diagnosis of hairy cell leukemia.
Description
ANXA1 Mouse Recombinant produced in E.Coli is a single,
non-glycosylated polypeptide chain containing 369 amino acids (1-346 a.a) and
having a molecular mass of 41.1kDa.
ANXA1 is fused to a 23 amino acid His-tag at N-terminus
& purified by proprietary chromatographic techniques.
Source
Escherichia Coli.
Physical Appearance
Sterile filtered colorless solution.
Formulation
ANXA1 protein solution (1mg/ml) containing Phosphate
buffered saline (pH7.4), 20% glycerol and 1mM DTT.
Stability
Store at 4°C if entire vial will be used within 2-4 weeks.
Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid multiple freeze-thaw cycles.
Purity
Greater than 95.0% as determined by SDS-PAGE.
Amino acid sequence
MGSSHHHHHH SSGLVPRGSH MGSMAMVSEF LKQARFLENQ EQEYVQAVKS YKGGPGSAVS PYPSFNVSSD
VAALHKAIMV KGVDEATIID ILTKRTNAQR QQIKAAYLQE NGKPLDEVLR KALTGHLEEV VLAMLKTPAQ
FDADELRGAM KGLGTDEDTL IEILTTRSNE QIREINRVYR EELKRDLAKD ITSDTSGDFR KALLALAKGD
RCQDLSVNQD LADTDARALY EAGERRKGTD VNVFTTILTS RSFPHLRRVF QNYGKYSQHD MNKALDLELK
GDIEKCLTTI VKCATSTPAF FAEKLYEAMK GAGTRHKALI RIMVSRSEID MNEIKVFYQK KYGISLCQAI
LDETKGDYEK ILVALCGGN.
Safety Data Sheet
Usage
ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
