Catalogue number
ANT-526
Synonyms
Tubulin folding cofactor B, Cytoskeleton-associated
protein CKAPI, cytoskeleton associated protein 1, CKAP1, CG22, Tubulin-specific
chaperone B.
Type
Mouse Anti Human Monoclonal.
Introduction
TBCB is a member of the Microtubules family. Biosynthesis of functional microtubules include the involvement of a number of chaperones, termed Tubulin folding cofactors A (TBCA), B (TBCB), C (TBCC), D (TBCD) and E (TBCE), which operate on folding intermediates downstream of the cytosolic chaperon, known as TCP. The 244 amino acid cytoplasmic protein TBCB has one CAP-Gly domain and is universally expressed. TBCB takes part in the regulation of tubulin heterodimer dissociation and operates as a negative regulator of axonal growth.
Clone
PAT1F6AT.
Physical Appearance
Sterile filtered colorless solution.
Immunogen
Anti-human TBCB mAb, clone
PAT1F6AT, is derived from hybridization of mouse F0 myeloma cells with spleen
cells from BALB/c mice immunized with a recombinant human TBCB protein 1-244 amino
acids purified from E. coli.
Ig Subclass
Mouse IgG2b heavy chain and k light chain.
Purification Method
TBCB antibody was purified from mouse ascitic fluids by
protein-A affinity chromatography.
Formulation
1mg/ml containing PBS, pH-7.4, 10% Glycerol and 0.02% Sodium Azide.
Storage Procedures
For periods up to 1 month store at 4°C, for longer periods of time, store at -20°C.?
Prevent freeze thaw cycles.
Stability / Shelf Life
12 months at -20°C. 1 month at 4°C.
Applications
The antibody has been tested by ELISA, Western blot
analysis to assure specificity and reactivity. Since application varies,
however, each investigation should be titrated by the reagent to obtain optimal
results. Recommended starting dilution is 1:1500.
Safety Data Sheet
Usage
ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
