Catalogue number
SARS-255
Introduction
SARS Coronavirus is an enveloped virus containing three outer structural proteins, namely the membrane (M), envelope (E), and spike (S) proteins. Spike (S)-glycoprotein of the virus interacts with a cellular receptor and mediates membrane fusion to allow viral entry into susceptible target cells. Accordingly, S-protein plays an important role in virus infection cycle and is the primary target of neutralizing antibodies.
Description
The E.Coli derived 35 kDa recombinant protein contains Nucleocapsid protein 1-49, 192-220 amino acids immunodominant regions. The 2 regions are separated with 3 glycine residues.
Purification Method
SARS Core protein was purified by proprietary chromatographic technique.
Purity
SARS Core protein is >95% pure as determined by 10% PAGE (coomassie staining).
Formulation
50mM Tris-HCl, 60mM NaCl and 50% glycerol.
Stability
SARS Core protein is shipped at ambient temperature.?
Upon arrival, store at -20C.
Specificity
SARS Core protein is Immunoreactive with sera of SARS-infected individuals.
Applications
SARS Core antigen is suitable for ELISA and Western blots, excellent antigen for detection of SARS with minimal specificity problems.
Safety Data Sheet
Usage
ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
