Catalogue number
PRO-1125
Synonyms
Ribosomal protein S3A, v-fos transformation effector protein 1, 40S ribosomal protein S3a, MFTL, FTE1, S3A.
Introduction
RPS3A is a member of the S3AE family of ribosomal proteins. Ribosomes, the organelles which catalyze protein synthesis, composed of a large 60S subunit and a small 40S subunit. Together these subunits consist of 4 RNA classes and around 80 structurally diverse proteins. RPS3A, a component of the 40S subunit, is a ribosomal protein that is situated in the cytoplasm. Interference of the gene encoding rat ribosomal protein S3a, in v-fos-transformed rat cells cause a degeneration of the transformed phenotype.
Description
RPS3A Human Recombinant produced in E. coli is a single polypeptide chain containing 288 amino acids (1-264) and having a molecular mass of 32.5kDa.
RPS3A is fused to a 24 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Source
E.coli.
Physical Appearance
Sterile Filtered colorless solution.
Formulation
The RPS3A solution (0.5mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 200mM NaCl, 2mM DTT and 50% glycerol.
Stability
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid multiple freeze-thaw cycles.
Purity
Greater than 90% as determined by SDS-PAGE.
Amino acid sequence
MGSSHHHHHH SSGLVPRGSH MGSHMAVGKN KRLTKGGKKG AKKKVVDPFS KKDWYDVKAP AMFNIRNIGK TLVTRTQGTK IASDGLKGRV FEVSLADLQN DEVAFRKFKL ITEDVQGKNC LTNFHGMDLT RDKMCSMVKK WQTMIEAHVD VKTTDGYLLR LFCVGFTKKR NNQIRKTSYA QHQQVRQIRK KMMEIMTREV QTNDLKEVVN KLIPDSIGKD IEKACQSIYP LHDVFVRKVK MLKKPKFELG KLMELHGEGS SSGKATGDET GAKVERADGY EPPVQESV.
Safety Data Sheet
Usage
ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
