Catalogue number
PRO-1494
Synonyms
ESE3, ESE3B, ESEJ, F-ATPase gamma subunit, ATP5C1, ATP5CL1, ATP5C, ATP synthase subunit gamma, mitochondrial.
Introduction
ATP synthase subunit gamma, mitochondrial isoform L (liver) (ATP5C1) is a member of the ATPase gamma chain family. ATP5C1 catalyzes ATP synthesis by using an electrochemical gradient of protons across the inner membrane throughout oxidative phosphorylation. The catalytic portion of mitochondrial ATP synthase is comprised of 5 different subunits (alpha, beta, gamma, delta, and epsilon) gathered with a stoichiometry of 3 alpha, 3 beta, and a single representative of the other 3.
Description
ATP5C1 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 296 amino acids (26-298 a.a.) and having a molecular mass of 32.6kDa.
ATP5C1 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Source
Escherichia Coli.
Physical Appearance
Sterile Filtered clear solution.
Formulation
ATP5C1 protein solution (0.5mg/ml) contains 20mM Tris-HCl buffer, (pH 8.0), 0.4M Urea and 10% glycerol.
Stability
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid multiple freeze-thaw cycles.
Purity
Greater than 90.0% as determined by SDS-PAGE.
Amino acid sequence
MGSSHHHHHH SSGLVPRGSH MGSATLKDIT RRLKSIKNIQ KITKSMKMVA AAKYARAERE LKPARIYGLG SLALYEKADI KGPEDKKKHL LIGVSSDRGL CGAIHSSIAK QMKSEVATLT AAGKEVMLVG IGDKIRGILY RTHSDQFLVA FKEVGRKPPT FGDASVIALE LLNSGYEFDE GSIIFNKFRS VISYKTEEKP IFSLNTVASA DSMSIYDDID ADVLQNYQEY NLANIIYYSL KESTTSEQSA RMTAMDNASK NASEMIDKLT LTFNRTRQAV ITKELIEIIS GAAALD.
Safety Data Sheet
Usage
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
