Catalogue number
HIV-002
Introduction
HIV-2 protease is very significant in the life cycle of the HIV virus. It is expressed in the infected cells as a part of Gag-Pol polyprotein from which it is auto-catalytycaly released after formation of an immature viral particle. The enzyme subsequently cleaves the other parts of viral polyproteins resulting in the maturation of the virus. In HIV-infected patients the enzyme is subjected to intensive mutagenesis and mutants resistant to applied medicines are produced as a result of the selection pressure.
Description
HIV-2 protease is an active homodimer having a molecular mass of 10.7kDa.
Source
Escherichia Coli.
Physical Appearance
Sterile filtered colorless clear solution.
Formulation
The HIV-2 Protease solution (0.25mg/1ml) is formulated in 20mM Tris pH7.0, 20mM MES, 200mM NaCl, 1mM EDT, 0.5mM DTT, 0.05% PEG 8000and 10% glycerol.
Storage Buffer
20mM HEPES, pH7.0 and 10% Glycerol.
Stability
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
Please avoid freeze thaw cycles.
Kinetic parameters
Km=740μM, Kcat = 30s-1, Kcat/Km=4.1mM-1s-1 with peptide substrate ATLNFPISPW.
Safety Data Sheet
Usage
ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
