Catalogue number
ENZ-1002
Synonyms
NMNAT, NMNAT1, PNAT1, Nicotinamide mononucleotide adenylyltransferase 1, NMN adenylyltransferase 1, Nicotinate-nucleotide adenylyltransferase 1, NaMN adenylyltransferase 1, EC=2.7.7.1, EC=2.7.7.18.
Introduction
NMNAT1 enzyme is vital for NAD biosynthesis, catalyzing the condensation of nicotinamide mononucleotide (NMN) or nicotinic acid mononucleotide (NaMN) with the AMP moiety of ATP to form NAD or NaAD. NMNAT1 is widely expressed with high levels in skeletal muscle, heart, liver and kidney. This protein appears to have the ability to protect against axonal degeneration following mechanical or toxic insults.
Description
NMNAT1 Recombinant Human produced in E.Coli is a single,
non-glycosylated polypeptide chain containing 315 amino acids (1-279 a.a.) and
having a molecular mass of 36 kDa.?
The NMNAT1 is fused to a 36 amino acid
His-Tag at N-terminus and purified by proprietary chromatographic techniques.
Source
Escherichia Coli.
Physical Appearance
Sterile filtered colorless solution.
Formulation
The NMNAT1 solution (1mg/ml) contains 20mM Tris-HCl
buffer (pH8.0), 20% glycerol, 0.1M NaCl, 1mM DTT and 1mM EDTA.
Stability
Store at 4°C if entire vial will be used within 2-4
weeks.
Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier
protein (0.1% HSA or BSA).
Avoid multiple freeze-thaw cycles.
Purity
Greater than 95.0% as determined by SDS-PAGE.
Biological Activity
Specific activity is > 7,000 pmol/min/ug, and was
obtained by measuring the beta-NAD from nicotinamide mononucleotide and ATP per
minute at pH 8.0 at 37C.
Amino acid sequence
MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSMENS EKTEVVLLAC GSFNPITNMH LRLFELAKDY MNGTGRYTVV KGIISPVGDA YKKKGLIPAY HRVIMAELAT KNSKWVEVDT WESLQKEWKE TLKVLRHHQE KLEASDCDHQ QNSPTLERPG RKRKWTETQD SSQKKSLEPK TKAVPKVKLL CGADLLESFA VPNLWKSEDI TQIVANYGLI CVTRAGNDAQ KFIYESDVLW KHRSNIHVVN EWIANDISST KIRRALRRGQSIRYLVPDLV QEYIEKHNLY SSESEDRNAG VILAPLQRNT AEAKT.
Safety Data Sheet
Usage
ProSpec's products are furnished for?LABORATORY RESEARCH?USE?ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
