Catalogue number
PKA-053
Synonyms
PI5P4KB, PIP5K2B, PIP5KIIB, PIP5KIIbeta, Phosphatidylinositol 5-phosphate 4-kinase type-2 beta, 1-phosphatidylinositol 5-phosphate 4-kinase 2-beta, Diphosphoinositide kinase 2-beta, Phosphatidylinositol 5-phosphate 4-kinase type II beta, PI(5)P 4-kinase type II beta, PIP4KII-beta, PtdIns(5)P-4-kinase isoform 2-beta, PIP4K2B.
Introduction
Phosphatidylinositol-5-Phosphate 4-Kinase, Type II, Beta (PIP4K2B) belongs to the phosphatidylinositol-4-phosphate 5-kinase family.PIP4K2B catalyzes the phosphorylation of phosphatidylinositol-4-phosphate on the 5th hydroxyl of the myo-inositol ring to form phosphatidylinositol-4,5-bisphosphate. PIP4K2B amino acid sequence does not show resemblance to another kinases although PIP4K2B presents kinase activity.PIP4K2B also interacts with p55 TNF receptor.
Description
PIP4K2B Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 439 amino acids (1-416 a.a) and having a molecular mass of 49.8kDa.PIP4K2B is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Source
Escherichia Coli.
Physical Appearance
Sterile Filtered clear solution.
Formulation
PIP4K2B protein solution (0.5mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 0.15M NaCl, 10% glycerol and 1mM DTT.
Stability
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid multiple freeze-thaw cycles.
Purity
Greater than 90.0% as determined by SDS-PAGE.
Amino acid sequence
MGSSHHHHHH SSGLVPRGSH MGSMSSNCTS TTAVAVAPLS ASKTKTKKKH FVCQKVKLFR ASEPILSVLM WGVNHTINEL SNVPVPVMLM PDDFKAYSKI KVDNHLFNKE NLPSRFKFKE YCPMVFRNLR ERFGIDDQDY QNSVTRSAPI NSDSQGRCGT RFLTTYDRRF VIKTVSSEDV AEMHNILKKY HQFIVECHGN TLLPQFLGMY RLTVDGVETY MVVTRNVFSH RLTVHRKYDL KGSTVAREAS DKEKAKDLPT FKDNDFLNEG QKLHVGEESK KNFLEKLKRD VEFLAQLKIM DYSLLVGIHD VDRAEQEEME VEERAEDEEC ENDGVGGNLL CSYGTPPDSP GNLLSFPRFF GPGEFDPSVD VYAMKSHESS PKKEVYFMAI IDILTPYDTK KKAAHAAKTV KHGAGAEIST VNPEQYSKRF NEFMSNILT.
Safety Data Sheet
Usage
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
