Catalogue number
PKA-059
Synonyms
Glucokinase, Glucose kinase, glk, b2388, JW2385.
Introduction
Glucokinase also known as GLK is a member of the bacterial glucokinase
family. GLK is not highly significant in E.coli since glucose is already transported into the cell through
the PTS system as glucose 6-phosphate.
Description
GLK E.coli Recombinant produced in E.Coli is a single,
non-glycosylated polypeptide chain containing 344 amino acids (1-321 a.a) and
having a molecular mass of 37.1kDa.
GLK is fused to a 23 amino acid His-tag at N-terminus
& purified by proprietary chromatographic techniques.
Source
E.coli.
Physical Appearance
Sterile Filtered colorless solution.
Formulation
GLK protein solution (1.0 mg/ml) containing 20mM Tris-HCl
buffer (pH 8.0), 0.15M NaCl and 10% glycerol.
Stability
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid multiple freeze-thaw cycles.
Purity
Greater than 95% as determined by SDS-PAGE.
Amino acid sequence
MGSSHHHHHH SSGLVPRGSH MGSMTKYALV GDVGGTNARL ALCDIASGEI SQAKTYSGLD YPSLEAVIRV
YLEEHKVEVK DGCIAIACPI TGDWVAMTNH TWAFSIAEMK KNLGFSHLEI INDFTAVSMA IPMLKKEHLI
QFGGAEPVEG KPIAVYGAGT GLGVAHLVHV DKRWVSLPGE GGHVDFAPNS EEEAIILEIL RAEIGHVSAE RVLSGPGLVN LYRAIVKADN
RLPENLKPKD ITERALADSC TDCRRALSLF CVIMGRFGGN LALNLGTFGG VFIAGGIVPR FLEFFKASGF
RAAFEDKGRF KEYVHDIPVY LIVHDNPGLL GSGAHLRQTL GHIL.
Safety Data Sheet
Usage
ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
