Catalogue number
ENZ-847
Synonyms
PAP, ACP3, ACP-3, Acid phosphatase, prostate, 5'-nucleotidase,
5'-NT, Ecto-5'-nucleotidase, Thiamine monophosphatase, TMPase.
Introduction
Acid phosphatase, prostate (ACPP) is a non-specific tyrosine phosphatase, which dephosphorylates a varied number of substrates under acidic conditions (pH 4-6) including alkyl, aryl, and acyl orthophosphate monoesters and phosphorylated proteins. ACPP has lipid phosphatase activity and inactivates lysophosphatidic acid in seminal plasma.
Description
ACPP Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 375 amino acids (33-386 a.a) and having a molecular mass of 43.2kDa.
ACPP is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Source
Escherichia Coli.
Physical Appearance
Sterile Filtered colorless solution.
Formulation
ACPP protein solution (0.5mg/ml) containing 20mM Tris-HCl
buffer (pH 8.0) and 10% glycerol.
Stability
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid multiple freeze-thaw cycles.
Purity
Greater than 85.0% as determined by SDS-PAGE.
Amino acid sequence
MGSSHHHHHH SSGLVPRGSH
MKELKFVTLV FRHGDRSPID TFPTDPIKES SWPQGFGQLT QLGMEQHYEL GEYIRKRYRK FLNESYKHEQ VYIRSTDVDR
TLMSAMTNLA ALVPPEGVSI WNPILLWQPI PVHTVPLSED QLLYLPFRNC PRFQELESET LKSEEFQKRL
HPYKDFIATL GKLSGLHGQD LFGIWSKVYD PLYCESVHNF TLPSRATEDT MTKLRELSEL SLLSLYGIHK
QKEKSRLQGG VLVNEILNHM KRATQIPSYK KLIMYSAHDT TVSGLQMALD VYNGLLPPYA SCHLTELYFE
KGEYFVEMYY RNETQHEPYP LMLPGCSPSC PLERFAELVG PVIPQDWSTE CMTTNSHQGT EDSTD.
Safety Data Sheet
Usage
ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
