Catalogue number
ENZ-829
Synonyms
Lysyl Oxidase, EC 1.4.3.13, Protein-lysine 6-oxidase, Lysyl
oxidase.
Introduction
Lysyl Oxidase also known as LOX?
is an extracellular copper enzyme which initiates the crosslinking of
collagens and? elastin. LOX catalyzes
oxidative deamination of the epsilon-amino group in certain lysine and
hydroxylysine residues of collagens and lysine residues of elastin. Adding up
to crosslinking extracellular matrix proteins, LOX plays a part in tumor
suppression. Moreover, defects in LOX are the cause of autosomal recessive
cutis laxa type I, CL type I. Two transcript variants encoding dissimilar isoforms
have been found for LOX.
Description
LOX Human Recombinant produced in E.Coli is a single,
non-glycosylated polypeptide chain containing 272 amino acids (169-417a.a) and
having a molecular mass of 31.4kDa.
LOX is fused to a 23 amino acid His-tag at N-terminus
& purified by proprietary chromatographic techniques.
Source
E.coli.
Physical Appearance
Sterile Filtered colorless solution.
Formulation
LOX protein solution (1mg/ml) containing 20mM Tris-HCl
buffer (pH 8.0) and 10% glycerol.
Stability
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid multiple freeze-thaw cycles.
Purity
Greater than 85% as determined by SDS-PAGE.
Amino acid sequence
MGSSHHHHHH SSGLVPRGSH MGSDDPYNPY KYSDDNPYYN YYDTYERPRP GGRYRPGYGT GYFQYGLPDL
VADPYYIQAS TYVQKMSMYN LRCAAEENCL ASTAYRADVR DYDHRVLLRF PQRVKNQGTS DFLPSRPRYS
WEWHSCHQHY HSMDEFSHYD LLDANTQRRV AEGHKASFCL
EDTSCDYGYH RRFACTAHTQ GLSPGCYDTY GADIDCQWID ITDVKPGNYI
LKVSVNPSYL VPESDYTNNV VRCDIRYTGH HAYASGCTIS PY.
Safety Data Sheet
Usage
ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
