Catalogue number
PKA-073
Synonyms
Mitogen-Activated Protein Kinase Kinase 6, PRKMK6, MEK6,
MKK6, Stress-Activated Protein Kinase Kinase 3,MAPK/ERK Kinase 6, SAPK Kinase
3, EC 2.7.12.2, MAPKK, SAPKK, SAPKK3, MEK 6, Protein Kinase, Mitogen-Activated,
Kinase 6 (MAP Kinase Kinase 6), Dual Specificity Mitogen-Activated Protein
Kinase Kinase 6, Kinase 6 (MAP Kinase Kinase 6), MAP Kinase Kinase
6,Mitogen-Activated, Protein Kinase, SKK3.
Introduction
Mitogen-Activated Protein Kinase 6 (MAP2K6) is a part of the dual specificity protein kinase family. MAP2K6 phosphorylates and activates p38 MAP kinase in response to inflammatory cytokines or environmental stress. MAP2K6 is a vital component of the MAP kinase signal transduction pathway. MAP2K6 takes part in various cellular processes such as stress induced cell cycle arrest and apoptosis.
Description
MAP2K6 Human Recombinant produced in E.Coli is a single,
non-glycosylated polypeptide chain containing 283 amino acids (53-314 a.a) and
having a molecular mass of 32kDa.MAP2K6 is fused to a 21 amino acid His-tag at N-terminus
& purified by proprietary chromatographic techniques.
Source
Escherichia Coli.
Physical Appearance
Sterile Filtered clear solution.
Formulation
MAP2K6 protein solution (0.25mg/ml) containing 20mM
Tris-HCl (pH8.0) and 10% glycerol.
Stability
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid multiple freeze-thaw cycles.
Purity
Greater than 85.0% as determined by SDS-PAGE.
Amino acid sequence
MGSSHHHHHH SSGLVPRGSH MLEPIMELGR GAYGVVEKMR HVPSGQIMAV KRIRATVNSQ EQKRLLMDLD ISMRTVDCPF
TVTFYGALFR EGDVWICMEL MDTSLDKFYK QVIDKGQTIP EDILGKIAVS IVKALEHLHS KLSVIHRDVK
PSNVLINALG QVKMCDFGIS GYLVDEVAKE IDAGCKPYMA PERINPELNQ KGYSVKSDIW SLGITMIELA
ILRFPYDSWG TPFQQLKQVV EEPSPQLPAD KFSAEFVDFT SQCLKKNSKE RPTYPELMQH PFF.
Safety Data Sheet
Usage
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
