BCOADC-E2 is a Full-length cDNA coding for the mature form of the human BCOADC-E2 protein having a molecular mass of 47,321 Dalton(pH 6.5). BCOADC-E2 protein is fused to a hexa-histidine purification tag.
Sf9 insect cells.
DLST (0.91mg/ml)?is supplied in 20mM HEPES buffer pH-8.0, 200mM NaCl and 20% glycerol.
1.?Binds IgG-type human auto-antibodies.
2.?Standard ELISA test (checkerboard analysis of positive/negative sera panels); immunodot test with positive/negative sera panels.
Western blot with?anti-M2-Antigen autoantibody-positive patient sera or?monoclonal
anti-hexa-His-tag antibody.
0.4-0.8 μg/ml (depending on the type of ELISA plate and coating buffer). Suitable for biotinylation and iodination.
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.?
Avoid multiple freeze-thaw cycles.
Greater than 75% as determined by SDS-PAGE.
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
