Catalogue number
ENZ-830
Synonyms
Glutamate Decarboxylase 1 (Brain, 67kDa), Glutamate
Decarboxylase 67 KDa Isoform, 67 KDa Glutamic Acid Decarboxylase, EC 4.1.1.15,
GAD-67, CPSQ1, SCP, GAD, Glutamate Decarboxylase 1 (Brain, 67kD), Glutamate
Decarboxylase 1, EC 4.1.1, GAD67, Glutamate decarboxylase 1.
Introduction
Glutamate Decarboxylase 1 Isoform-1, also known as GAD1 iso1 belongs to
the group II decarboxylase family. GAD1 iso1 encodes one of several forms of
glutamic acid decarboxylase, and is identified as a major autoantigen in
insulin-dependent diabetes. GAD1 iso1 is in charge for catalyzing the
production of gamma-aminobutyric acid from L-glutamic acid. In addition, a
pathogenic function for GAD1 iso1 has been shown in the human pancreas while it
has been identified as an autoantigen & an autoreactive T cell target in
insulin-dependent diabetes. GAD1 iso1 play a role in the stiff man syndrome. It
has been shown that deficiency in this enzyme has led to pyridoxine dependency
with seizures.
Description
GAD1 iso1 Human Recombinant produced in E.Coli is a single,
non-glycosylated polypeptide chain containing 617 amino acids (1-594 a.a) and
having a molecular mass of 69.3kDa.
GAD1 iso1 is fused to a 23 amino acid His-tag at
N-terminus & purified by proprietary chromatographic techniques.
Source
E.coli.
Physical Appearance
Sterile Filtered colorless solution.
Formulation
GAD1 iso1 protein solution (0.5mg/ml) containing 20mM Tris-HCl buffer (pH 8.0) and 10% glycerol.
Stability
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid multiple freeze-thaw cycles.
Purity
Greater than 80% as determined by SDS-PAGE.
Amino acid sequence
MGSSHHHHHH SSGLVPRGSH MGSMASSTPS SSATSSNAGA DPNTTNLRPT TYDTWCGVAH GCTRKLGLKI
CGFLQRTNSL EEKSRLVSAF KERQSSKNLL SCENSDRDAR FRRTETDFSN LFARDLLPAK NGEEQTVQFL
LEVVDILLNY VRKTFDRSTK VLDFHHPHQL LEGMEGFNLE
LSDHPESLEQ ILVDCRDTLK YGVRTGHPRF FNQLSTGLDI IGLAGEWLTS
TANTNMFTYE IAPVFVLMEQ ITLKKMREIV GWSSKDGDGI FSPGGAISNM YSIMAARYKY FPEVKTKGMA
AVPKLVLFTS EQSHYSIKKA GAALGFGTDN VILIKCNERG KIIPADFEAK ILEAKQKGYV
PFYVNATAGT TVYGAFDPIQ EIADICEKYN LWLHVDAAWG GGLLMSRKHR
HKLNGIERAN SVTWNPHKMM GVLLQCSAIL VKEKGILQGC NQMCAGYLFQ PDKQYDVSYD TGDKAIQCGR
HVDIFKFWLM WKAKGTVGFE NQINKCLELA EYLYAKIKNR EEFEMVFNGE PEHTNVCFWY
IPQSLRGVPD SPQRREKLHK
VAPKIKALMM ESGTTMVGYQ PQGDKANFFR MVISNPAATQ SDIDFLIEEI ERLGQDL.
Safety Data Sheet
Usage
ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
