Catalogue number
ENZ-824
Synonyms
Citrate Synthase, EC 2.3.3.1, Citrate (Si)-Synthase, EC
2.3.3, Citrate synthase, mitochondrial.
Introduction
Citrate synthase also known as CS is a Krebs tricarboxylic acid cycle
enzyme which catalyzes the synthesis of citrate from oxaloacetate and acetyl
coenzyme A. CS is present in almost all cells capable of oxidative metabolism.
CS is nuclear encoded and transported into the mitochondrial matrix, where the
mature form is found. The diseases related to CS are: critical illness
polyneuropathy and mitochondrial cardiomyopathy.
Description
CS Human Recombinant produced in E.Coli is a single,
non-glycosylated polypeptide chain containing 462 amino acids (28-466 a.a) and
having a molecular mass of 51.4kDa.
CS is fused to a 23 amino acid His-tag at N-terminus
& purified by proprietary chromatographic techniques.
Source
Escherichia Coli.
Physical Appearance
Sterile Filtered colorless solution.
Formulation
CS protein solution (1mg/ml) containing 20mM Tris-HCl
buffer (pH 8.0), 0.15M NaCl,?10% glycerol and 1mM DTT.
Stability
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid multiple freeze-thaw cycles.
Purity
Greater than 90.0% as determined by SDS-PAGE.
Amino acid sequence
MGSSHHHHHH SSGLVPRGSH MGSASSTNLK DILADLIPKE QARIKTFRQQ HGKTVVGQIT VDMMYGGMRG
MKGLVYETSV LDPDEGIRFR GFSIPECQKL LPKAKGGEEP LPEGLFWLLV TGHIPTEEQV SWLSKEWAKR
AALPSHVVTM LDNFPTNLHP MSQLSAAVTA LNSESNFARA YAQGISRTKY WELIYEDSMD LIAKLPCVAA KIYRNLYREG SGIGAIDSNL
DWSHNFTNML GYTDHQFTEL TRLYLTIHSD HEGGNVSAHT SHLVGSALSD PYLSFAAAMN GLAGPLHGLA
NQEVLVWLTQ LQKEVGKDVS DEKLRDYIWN TLNSGRVVPG YGHAVLRKTD PRYTCQREFA LKHLPNDPMF KLVAQLYKIV PNVLLEQGKA KNPWPNVDAH SGVLLQYYGM
TEMNYYTVLF GVSRALGVLA QLIWSRALGF PLERPKSMST EGLMKFVDSK SG.
Safety Data Sheet
Usage
ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
