Catalogue number
PKA-005
Synonyms
Deoxythymidylate
Kinase (Thymidylate Kinase), DTMP Kinase, CDC8, TMPK, TYMK, Thymidylate (DTMP)
Kinase, EC 2.7.4.9, PP3731, Thymidylate kinase.
Introduction
Thymidylate kinase (DTYMK) is involved in pyrimidine metabolism. Specifically, DTYMK catalyzes the ATP-dependent conversion of dTMP (deoxythymidine monophosphate) to dTDP (deoxythymidine diphosphate), which then acts as one of the 4 nucleotides in DNA. Through its role in the catalytic creation of dTDP, DTYMK has an imperative role in the pathway of DNA synthesis and is assumed to be involved in cell cycle progression and cell growth.
Description
DTYMK Human Recombinant produced in E.Coli is a single,
non-glycosylated polypeptide chain containing 232 amino acids (1-212 a.a) and
having a molecular mass of 26kDa.
DTYMK is fused to a 20
amino acid His-tag at N-terminus & purified by proprietary chromatographic
techniques.
Source
E.coli.
Physical Appearance
Sterile Filtered colorless solution.
Formulation
DTYMK
protein solution (1mg/ml) containing 20mM Tris-HCl buffer (pH 8.0), 20%
glycerol, 2mM DTT and 0.1M NaCl.
Stability
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid multiple freeze-thaw cycles.
Purity
Greater than 95% as determined by SDS-PAGE.
Amino acid sequence
MGSSHHHHHH SSGLVPRGSH
MAARRGALIV LEGVDRAGKS TQSRKLVEAL CAAGHRAELL RFPERSTEIG KLLSSYLQKK SDVEDHSVHL
LFSANRWEQV PLIKEKLSQG VTLVVDRYAF SGVAFTGAKE NFSLDWCKQP DVGLPKPDLV LFLQLQLADA
AKRGAFGHER YENGAFQERA LRCFHQLMKD TTLNWKMVDA SKSIEAVHED IRVLSEDAIR TATEKPLGEL WK.
Safety Data Sheet
Usage
ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
