Catalogue number
PKA-069
Synonyms
V-Akt Murine Thymoma Viral Oncogene Homolog 1, Protein
Kinase B Alpha, Proto-Oncogene C-Akt, RAC-PK-Alpha, EC 2.7.11.1, PKB Alpha,
CWS6, PKB, RAC, RAC-Alpha Serine/Threonine-Protein Kinase, Rac Protein Kinase
Alpha, Protein Kinase B, PKB-ALPHA, RAC-ALPHA, EC 2.7.11, AKT1m, PRKBA, AKT,
RAC-alpha serine/threonine-protein kinase.
Introduction
Akt1, also known as Akt or else protein kinase B (PKB) is an important
molecule in mammaliancellular signaling. In humans, there are three genes in
the "Akt family": Akt1, Akt2, and Akt3. Moreover, these enzymes are
members of the serine/threonine-specific protein kinase family (EC2.7.11.1). Akt1
is involved in cellular survival pathways, by inhibiting apoptoticprocesses.
Akt1 is also able to induce protein synthesis pathways, and is therefore a key
signaling protein in the cellular pathways which lead to skeletal muscle
hypertrophy, and general tissue growth. Since it can block apoptosis, and
thereby promote cell survival, Akt1 has been implicated as a most important
factor in numerous types of cancer. Akt (now also called Akt1) was at first
identified as the oncogenein the transforming retrovirus, AKT8.
Description
AKT1 produced in Sf9 Baculovirus cells is a single,
glycosylated polypeptide chain containing 488 amino acids (1-480a.a.) and
having a molecular mass of 56.7kDa. (Molecular size on SDS-PAGE will appear at
approximately 50-70kDa).
AKT1 is expressed with an 8 amino acid His tag at
C-Terminus and purified by proprietary chromatographic techniques.
Source
Sf9,
Baculovirus cells.
Physical Appearance
Sterile filtered colorless solution.
Formulation
AKT1 protein solution (0. 5mg/ml) contains phosphate
buffered saline (pH7.4) and 20% glycerol.
Stability
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid multiple freeze-thaw cycles.
Purity
Greater than 90.0% as determined by SDS-PAGE.
Amino acid sequence
MSDVAIVKEG WLHKRGEYIK TWRPRYFLLK NDGTFIGYKE RPQDVDQREA
PLNNFSVAQC QLMKTERPRP NTFIIRCLQW TTVIERTFHV ETPEEREEWT TAIQTVADGL KKQEEEEMDF RSGSPSDNSG
AEEMEVSLAK PKHRVTMNEF EYLKLLGKGT FGKVILVKEK ATGRYYAMKI
LKKEVIVAKD EVAHTLTENR VLQNSRHPFL TALKYSFQTH DRLCFVMEYA
NGGELFFHLS RERVFSEDRA RFYGAEIVSA LDYLHSEKNV VYRDLKLENL MLDKDGHIKI TDFGLCKEGI
KDGATMKTFC GTPEYLAPEV LEDNDYGRAV DWWGLGVVMY EMMCGRLPFY NQDHEKLFEL
ILMEEIRFPR TLGPEAKSLL SGLLKKDPKQ RLGGGSEDAK EIMQHRFFAG
IVWQHVYEKK LSPPFKPQVT SETDTRYFDE EFTAQMITIT PPDQDDSMEC VDSERRPHFP QFSYSASGTA LEHHHHHH.
Safety Data Sheet
Usage
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
