Catalogue number
ENZ-1008
Synonyms
Alpl,
Akp-2, Akp2, ALP, APTNAP, TNAP, TNSALP, HOPS, AP-TNAP, Alkaline phosphatase 2.
Introduction
Alkaline
Phosphatase Liver/Bone/Kidney (Alpl) is a part of the alkaline phosphatases
family which comprises 4 related alkaline phosphatases. Alpl is a
membrane-bound glycosylated enzyme which is not expressed in any particular
tissue. Alpl takes part in skeletal mineralization.
Description
ALPL produced in Sf9 Baculovirus cells is a single,
glycosylated polypeptide chain containing 493 amino acids (19-503 a.a.) and
having a molecular mass of 54.5kDa (Molecular size on SDS-PAGE will appear at
approximately 50-70kDa). ALPL is expressed with an 8 amino acid His tag at
C-Terminus and purified by proprietary chromatographic techniques.
Source
Sf9, Baculovirus cells.
Physical Appearance
Sterile Filtered colorless solution.
Formulation
ALPL protein solution (0.5mg/ml) contains Phosphate
Buffered Saline (pH 7.4) and 10% glycerol.
Stability
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid multiple freeze-thaw cycles.
Purity
Greater
than 95.0% as determined by SDS-PAGE.
Biological Activity
Specific activity is > 46,000 pmol/min/ug and is
defined as the amount of enzyme that hydrolyze 1pmole of 4-Methylumbelliferyl phosphate
to phosphate and 4-Methylumbelliferone per minute at pH 8.8 at 25C.
Amino acid sequence
VPEKERDPSY WRQQAQETLK NALKLQKLNT NVAKNVIMFL GDGMGVSTVT
AARILKGQLH HNTGEETRLE MDKFPFVALS KTYNTNAQVP DSAGTATAYL CGVKANEGTV GVSAATERTR
CNTTQGNEVT SILRWAKDAG KSVGIVTTTR VNHATPSAAY AHSADRDWYS DNEMPPEALS QGCKDIAYQL
MHNIKDIDVI MGGGRKYMYP KNRTDVEYEL DEKARGTRLD GLDLISIWKS FKPRHKHSHY VWNRTELLAL
DPSRVDYLLG LFEPGDMQYE LNRNNLTDPS LSEMVEVALR ILTKNLKGFF LLVEGGRIDH GHHEGKAKQA
LHEAVEMDQA IGKAGAMTSQ KDTLTVVTAD HSHVFTFGGY TPRGNSIFGL APMVSDTDKK PFTAILYGNG
PGYKVVDGER ENVSMVDYAH NNYQAQSAVP LRHETHGGED VAVFAKGPMA HLLHGVHEQN YIPHVMAYAS
CIGANLDHCA WAGSGLEHHH HHH.
Safety Data Sheet
Usage
ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
