Catalogue number
ENZ-275
Synonyms
Bacterial leucyl aminopeptidase, EC 3.4.11.10.
Description
The 29 kDa Aeromonas Aminopeptidase is produced by genetic engineering and can be used for physical & structural investigations, sequence and amino-terminal determinations. This exopeptidase recognizes a specific stop sign at –X- Pro and requires a free a-amino group in the L-configuration. It is therefore suitable for the removal of the redundant N-terminal methionine often added to engineered recombinant proteins.
Source
Aeromonas Proteolytica.
Physical Appearance
Sterile filtered liquid formulation.
Formulation
Buffered solution containing 10mM Tris-HCl, 100mM NaCl and 5μM ZnSO4, pH 8.0.
Stability
Two years when stored at -20°C, 2 weeks at 4°C.
Purity
Greater than 95.0% as determined by SEC-HPLC.
Biological Activity
Recombinant Aeromonas Aminopeptidase was found to have an activity of 108 Units/mg protein.
Unit Definition
One unit of aminopeptidase activity is defined as the amount of enzyme that releases 1 μmole p-nitroaniline at 25°C in 1 minute.
Safety Data Sheet
Usage
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
