Catalogue number
ENZ-921
Synonyms
Fucosidase, Alpha-L- 1, Tissue, Alpha-L-Fucoside
Fucohydrolase 1, Alpha-L-Fucosidase 1,
Alpha-L-Fucosidase I, EC 3.2.1.51, Tissue
Alpha-L-Fucosidase, EC 3.2.1, FUCA, Tissue alpha-L-fucosidase.
Introduction
Fucosidase Alpha-L- 1 Plasma, also
known as FUCA1 is a member of the glycosyl hydrolase 29 family which is
responsible for hydrolyzing the alpha-1,6-linked fucose joined to the reducing-end N-acetylglucosamine of the
carbohydrate moieties of glycoproteins. Fucosidosis is an autosomal recessive
lysosomal storage disease caused by the absence of alpha-L-fucosidase activity.
Description
FUCA1 produced in Sf9 Baculovirus cells is a single,
glycosylated polypeptide chain containing 445 amino acids (28-466a.a.) and
having a molecular mass of 51.7kDa.?
(Molecular size on SDS-PAGE will appear at
approximately 50-70kDa).?
FUCA1 is expressed with a 6 amino acid His tag at
C-Terminus and purified by proprietary chromatographic techniques.
Source
Sf9,
Baculovirus cells.
Physical Appearance
Sterile Filtered colorless solution.
Formulation
FUCA1 protein solution (0.5mg/ml) contains Phosphate
Buffered Saline (pH 7.4) and 10% glycerol.
Stability
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid multiple freeze-thaw cycles.
Purity
Greater than 90% as determined by SDS-PAGE.
Amino acid sequence
VRRAQPPRRY TPDWPSLDSR PLPAWFDEAK FGVFIHWGVF SVPAWGSEWF
WWHWQGEGRP QYQRFMRDNY PPGFSYADFG PQFTARFFHP EEWADLFQAA GAKYVVLTTK HHEGFTNWPS
PVSWNWNSKD VGPHRDLVGE LGTALRKRNI RYGLYHSLLE WFHPLYLLDK KNGFKTQHFV SAKTMPELYD
LVNSYKPDLI WSDGEWECPD TYWNSTNFLS WLYNDSPVKD EVVVNDRWGQ NCSCHHGGYY NCEDKFKPQS
LPDHKWEMCT SIDKFSWGYR RDMALSDVTE ESEIISELVQ TVSLGGNYLL NIGPTKDGLI VPIFQERLLA
VGKWLSINGE AIYASKPWRV QWEKNTTSVW YTSKGSAVYA IFLHWPENGV LNLESPITTS TTKITMLGIQ
GDLKWSTDPD KGLFISLPQL PPSAVPAEFA WTIKLTGVKH HHHHH.
Safety Data Sheet
Usage
ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
