Catalogue number
PKA-065
Synonyms
Cyclin-Dependent Kinase 1, CDC2, Cell Division Cycle 2,
G1 To S And G2 To M, Cell Division Control Protein 2 Homolog, Cell Division
Protein Kinase 1, P34 Protein Kinase, P34CDC2, CDC28A, Cell Cycle Controller
CDC2, EC 2.7.11.22, EC 2.7.11.23, CDKN1, Cyclin-dependent kinase 1.
Introduction
Cyclin-dependent kinase 1 (CDK1) plays a significant part in the control
of the eukaryotic cell cycle through modulating the centrosome cycle in
addition to mitotic onset; CDK1 promotes G2-M transition and regulates G1
progress and G1-S transition using association with multiple interphase
cyclins. CDK1 is essential in higher cells for the entry into S-phase and
mitosis.
Description
CDK1 produced in Sf9 Baculovirus cells is a single,
glycosylated polypeptide chain containing 305 amino acids (1-297a.a.) and
having a molecular mass of 35.1kDa.
CDK1 is expressed with an 8 amino acid His tag at
C-Terminus and purified by proprietary chromatographic techniques.
Source
Sf9,
Baculovirus cells.
Physical Appearance
Sterile filtered colorless solution.
Formulation
CDK1 protein solution (0.5mg/ml) contains Phosphate buffered saline (pH7.4), 30% glycerol, 2mM DTT and 0.1mM PMSF.
Stability
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid multiple freeze-thaw cycles.
Purity
Greater than 95.0% as determined by SDS-PAGE.
Amino acid sequence
MEDYTKIEKI GEGTYGVVYK GRHKTTGQVV AMKKIRLESE EEGVPSTAIR
EISLLKELRH PNIVSLQDVL MQDSRLYLIF EFLSMDLKKY LDSIPPGQYM DSSLVKSYLY QILQGIVFCH
SRRVLHRDLK PQNLLIDDKG TIKLADFGLA RAFGIPIRVY THEVVTLWYR SPEVLLGSAR
YSTPVDIWSI GTIFAELATK KPLFHGDSEI DQLFRIFRAL GTPNNEVWPE
VESLQDYKNT FPKWKPGSLA SHVKNLDENG LDLLSKMLIY DPAKRISGKM ALNHPYFNDL DNQIKKMLEH
HHHHH.
Safety Data Sheet
Usage
ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
