Catalogue number
PKA-076
Synonyms
Cyclin-Dependent Kinase 1, CDC2, Cell Division Cycle 2,
G1 To S And G2 To M, Cell Division Control Protein 2 Homolog, Cell Division
Protein Kinase 1, P34 Protein Kinase, P34CDC2, CDC28A, Cell Cycle Controller
CDC2, EC 2.7.11.22, EC 2.7.11.23, CDKN1, Cyclin-dependent kinase 1, CDK1.
Introduction
Cyclin-dependent kinase 1 (CDK1) plays a significant part in the control of the eukaryotic cell cycle through modulating the centrosome cycle in addition to mitotic onset; CDK1 promotes G2-M transition and regulates G1 progress and G1-S transition using association with multiple interphase cyclins. CDK1 is essential in higher cells for the entry into S-phase and mitosis.
Description
CDK1 Human Recombinant produced in E.Coli is a single, non-glycosylated
polypeptide chain containing 317 amino acids (1-297 a.a) and having a molecular
mass of 36.2kDa.
CDK1 is fused to a 20 amino
acid His-tag at N-terminus & purified by proprietary chromatographic
techniques.
Source
Escherichia Coli.
Physical Appearance
Sterile filtered colorless solution.
Formulation
CDK1
protein solution (1mg/ml) containing 20mM Tris-HCl (pH8.0) and 10% glycerol.
Stability
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid multiple freeze-thaw cycles.
Purity
Greater than 85.0% as determined by SDS-PAGE.
Amino acid sequence
MGSSHHHHHH SSGLVPRGSH
MEDYTKIEKI GEGTYGVVYK GRHKTTGQVV AMKKIRLESE EEGVPSTAIR EISLLKELRH PNIVSLQDVL
MQDSRLYLIF EFLSMDLKKY LDSIPPGQYM DSSLVKSYLY QILQGIVFCH SRRVLHRDLK PQNLLIDDKG
TIKLADFGLA RAFGIPIRVY THEVVTLWYR SPEVLLGSAR YSTPVDIWSI GTIFAELATK KPLFHGDSEI
DQLFRIFRAL GTPNNEVWPE VESLQDYKNT FPKWKPGSLA SHVKNLDENG LDLLSKMLIY DPAKRISGKM
ALNHPYFNDL DNQIKKM.
Safety Data Sheet
Usage
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
