Catalogue number
ENZ-912
Synonyms
Glutaminyl-Peptide Cyclotransferase, Glutaminyl Cyclase, QC,
Glutaminyl-TRNA Cyclotransferase, Glutamyl
Cyclase, EC 2.3.2.5, SQC, EC, GCT, Glutaminyl-peptide cyclotransferase.
Introduction
Glutaminyl-Peptide Cyclotransferase, also known as QPCT is a member of
the glutaminyl-peptide cyclotransferase family. QPCT is responsible for the
biosynthesis of pyroglutamyl peptides. Furthermore, QPCT is partial against
acidic and tryptophan residues adjacent to the N-terminal glutaminyl residue
and a lack of importance of chain length following the second residue. QPCT
catalyzes N-terminal pyroglutamate formation, also in vitro, it catalyzes
pyroglutamate formation of N-terminally truncated form of APP amyloid-beta
peptides [Glu-3]-beta-amyloid.
Description
QPCT produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 339 amino acids (29-361a.a.) and having a molecular mass of 38.7kDa (Molecular size on SDS-PAGE will appear at approximately 28-40kDa). QPCT is expressed with a 6 amino acid His tag at C-Terminus and purified by proprietary chromatographic techniques.
Source
Sf9,
Baculovirus cells.
Physical Appearance
Sterile Filtered colorless solution.
Formulation
QPCT protein solution (0.5mg/ml) contains Phosphate
Buffered Saline (pH 7.4) and 10% glycerol.
Stability
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid multiple freeze-thaw cycles.
Purity
Greater than 90.0% as determined by SDS-PAGE.
Amino acid sequence
VSPSASAWPE EKNYHQPAIL NSSALRQIAE GTSISEMWQN DLQPLLIERY
PGSPGSYAAR QHIMQRIQRL QADWVLEIDT FLSQTPYGYR SFSNIISTLN PTAKRHLVLA CHYDSKYFSH
WNNRVFVGAT DSAVPCAMML ELARALDKKL LSLKTVSDSK PDLSLQLIFF DGEEAFLHWS
PQDSLYGSRH LAAKMASTPH PPGARGTSQL HGMDLLVLLD LIGAPNPTFP
NFFPNSARWF ERLQAIEHEL HELGLLKDHS LEGRYFQNYS YGGVIQDDHI PFLRRGVPVL HLIPSPFPEV
WHTMDDNEEN LDESTIDNLN KILQVFVLEY LHLHHHHHH.
Safety Data Sheet
Usage
ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
