Catalogue number
ENZ-461
Synonyms
MAT2-beta, MAT-2B, MAT2-B, DTDP-4-keto-6-deoxy-D-glucose 4-reductase, MAT-II, MATIIbeta, MAT II beta, Methionine adenosyltransferase 2 subunit beta, Methionine adenosyltransferase II beta, MGC12237, MSTP045, Nbla02999, SDR23E1, TGR, UNQ2435/PRO4995, MAT2B.
Introduction
MAT2B is part of the methionine adenosyltransferase family. MAT2B catalyzes the biosynthesis of S-adenosylmethionine from methionine and ATP. MAT2B is the regulatory beta subunit of MAT. MAT2B expression in hepatoma cell lines increases DNA synthesis and thus take part in cell proliferation.
Description
Recombinant Human MAT2B produced in E.Coli is a single, non-glycosylated polypeptide chain containing 323 amino acids (1-323 a.a) and having a molecular mass of 36.4 kDa.
MAT2B is is purified by conventional chromatography techniques.
Source
Escherichia Coli.
Physical Appearance
Sterile filtered colorless solution.
Formulation
The MAT2B protein solution contains 20mM Tris-HCl, pH-8, 1mM DTT, 1mM EDTA and 10% Glycerol.
Stability
Store at 4°C if entire vial will be used within 2-4 weeks.
Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid multiple freeze-thaw cycles.
Purity
Greater than 90.0% as determined by SDS-PAGE.
Amino acid sequence
MPEMPEDMEQ EEVNIPNRRV LVTGATGLLG RAVHKEFQQN NWHAVGCGFR RARPKFEQVN LLDSNAVHHI IHDFQPHVIV HCAAERRPDV VENQPDAASQ LNVDASGNLA KEAAAVGAFL IYISSDYVFD GTNPPYREED IPAPLNLYGK TKLDGEKAVL ENNLGAAVLR IPILYGEVEK LEESAVTVMF DKVQFSNKSA NMDHWQQRFP THVKDVATVC RQLAEKRMLD PSIKGTFHWS GNEQMTKYEM ACAIADAFNL PSSHLRPITD SPVLGAQRPR NTQLDCSKLE TLGIGQRTPF RIGIKESLWP FLIDKRWRQT VFH.
Safety Data Sheet
Usage
ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
