Catalogue number
ENZ-820
Synonyms
Farnesyl-Diphosphate Farnesyltransferase 1, Squalene
Synthase, SQS, SS, FPP:FPP Farnesyltransferase, EC 2.5.1.21, DGPT, ERG9, Presqualene-Di-Diphosphate
Synthase, Squalene Synthetase, Farnesyl-Diphosphate, Farnesyltransferase, FDFT1.
Introduction
Farnesyl-Diphosphate Farnesyltransferase 1, also known as FDFT1 is a
membrane-associated enzyme sited at a branch point in the mevalonate pathway. FDFT1
belongs to the phytoene/squalene synthase family. FDFT1 is the 1st specific
enzyme in cholesterol biosynthesis, which catalyzes the dimerization of 2
molecules of farnesyl diphosphate in a two-step reaction to form squalene.
Description
FDFT1 Human Recombinant produced in E.Coli is a single,
non-glycosylated polypeptide chain containing 306 amino acids (1-283 a.a) and
having a molecular mass of 35.4 kDa.?
FDFT1 is fused to a 23 amino acid His-tag at N-terminus
& purified by proprietary chromatographic techniques.
Source
Escherichia Coli.
Physical Appearance
Sterile Filtered colorless solution.
Formulation
FDFT1 protein solution (0.25 mg/ml) containing 20mM
Tris-HCl buffer (pH 8.0), 10% glycerol and 0.4M Urea.
Stability
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid multiple freeze-thaw cycles.
Purity
Greater than 85.0% as determined by SDS-PAGE.
Amino acid sequence
MGSSHHHHHH SSGLVPRGSH MGSMEFVKCL GHPEEFYNLV RFRIGGKRKV MPKMDQDSLS SSLKTCYKYL
NQTSRSFAAV IQALDGEMRN AVCIFYLVLR ALDTLEDDMT ISVEKKVPLL HNFHSFLYQP DWRFMESKEK
DRQVLEDFPT ISLEFRNLAE KYQTVIADIC RRMGIGMAEF
LDKHVTSEQE WDKYCHYVAG
LVGIGLSRLF SASEFEDPLV GEDTERANSM GLFLQKTNII RDYLEDQQGG REFWPQEVWS RYVKKLGDFA KPENIDLAVQ
CLNELITNAL HHIPDVITYL SRLRNQ.
Safety Data Sheet
Usage
ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
