Catalogue number
PKA-091
Synonyms
Serine/Threonine Kinase 3, Mammalian STE20-like protein
kinase 2, MST-2, STE20-like kinase MST2, Serine/threonine-protein kinase Krs-1,
MST2/N, MST2/C, KRS1,MST2.
Introduction
Serine/threonine-protein kinase 3 (), is a mammalian Ste20-related
protein kinases most closely related to Drosophila Hippo, which is a major regulator
of cell proliferation as well as survival in the course of development.? STK3 is a serine/threonine kinase which
functions early in a pheromone responsive signal transduction cascade in yeast.
Furthermore, STK3 activates the human large tumor suppressor kinase Lats1. STK3
also modulates stress-induced cardiac hypertrophy.
Description
STK3 Human Recombinant produced in Sf9 Insect cell is a
single, non-glycosylated polypeptide chain containing 331 amino acids (1-322aa.a)
and having a molecular mass of 37.6kDa (Migrates at 40-57kDa on SDS-PAGE under
reducing conditions).
STK3 is fused to a 6 amino acid
His tag at C-Terminus and purified by proprietary chromatographic techniques.
Source
Sf9 Insect cells.
Physical Appearance
Sterile Filtered colorless solution.
Formulation
STK3 protein solution (0.25mg/ml) containing Phosphate
Buffered Saline (pH 7.4) and 10% glycerol.
Stability
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid multiple freeze-thaw cycles.
Purity
Greater than 90% as determined by SDS-PAGE.
Amino acid sequence
ADPMEQPPAP KSKLKKLSED
SLTKQPEEVF DVLEKLGEGS YGSVFKAIHK ESGQVVAIKQ VPVESDLQEI IKEISIMQQC DSPYVVKYYG SYFKNTDLWI
VMEYCGAGSV SDIIRLRNKT LIEDEIATIL KSTLKGLEYL HFMRKIHRDI KAGNILLNTE GHAKLADFGV
AGQLTDTMAK RNTVIGTPFW MAPEVIQEIG YNCVADIWSL GITSIEMAEG KPPYADIHPM RAIFMIPTNP PPTFRKPELW SDDFTDFVKK CLVKNPEQRA TATQLLQHPF IKNAKPVSIL RDLITEAMEI KAKRHEEQQR ELEEEEENSD
EDELDHHHHH H.
Safety Data Sheet
Usage
ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
