Catalogue number
ENZ-887
Synonyms
Sulfite Oxidase, EC 1.8.3.1, Sulfite oxidase,
mitochondrial.
Introduction
Sulfite oxidase, also known as SUOX is a homodimeric protein localized
to the intermembrane space of mitochondria. Each subunit includes a heme domain
as well as a molybdopterin-binding domain. The SUOX enzyme catalyzes the
oxidation of sulfite to sulfate, the last reaction in the oxidative degradation
of the sulfur amino acids cysteine and methionine. In addition, the deficiency
of SUOX results in neurological abnormalities which are often fatal at an early
age.
Description
SUOX Human Recombinant produced in E.Coli is a single, non-glycosylated
polypeptide chain containing 489 amino acids (80-545 a.a) and having a
molecular mass of 53.9kDa.
SUOX is fused to a 23 amino acid His-tag at N-terminus
& purified by proprietary chromatographic techniques.
Source
E.coli.
Physical Appearance
Sterile Filtered colorless solution.
Formulation
SUOX protein solution (1mg/ml) containing Phosphate
buffered saline (pH7.4), 30% glycerol and 1mM DTT.
Stability
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid multiple freeze-thaw cycles.
Purity
Greater than 90% as determined by SDS-PAGE.
Amino acid sequence
MGSSHHHHHH SSGLVPRGSH MGSESTHIYT KEEVSSHTSP ETGIWVTLGS EVFDVTEFVD LHPGGPSKLM
LAAGGPLEPF WALYAVHNQS HVRELLAQYK IGELNPEDKV APTVETSDPY ADDPVRHPAL KVNSQRPFNA
EPPPELLTEN YITPNPIFFT RNHLPVPNLD PDTYRLHVVG APGGQSLSLS LDDLHNFPRY EITVTLQCAG
NRRSEMTQVK EVKGLEWRTG AISTARWAGA RLCDVLAQAG HQLCETEAHV CFEGLDSDPT GTAYGASIPL
ARAMDPEAEV LLAYEMNGQP LPRDHGFPVR VVVPGVVGAR HVKWLGRVSV QPEESYSHWQ RRDYKGFSPS
VDWETVDFDS APSIQELPVQ SAITEPRDGE TVESGEVTIK GYAWSGGGRA VIRVDVSLDG GLTWQVAKLD
GEEQRPRKAW AWRLWQLKAP VPAGQKELNI VCKAVDDGYN VQPDTVAPIW NLRGVLSNAW HRVHVYVSP.
Safety Data Sheet
Usage
ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
