Catalogue number
PKA-086
Synonyms
|
Epidermal Growth Factor
Receptor, Receptor Tyrosine-Protein Kinase ErbB-1, Erb-B2 Receptor Tyrosine
Kinase, Proto-Oncogene C-ErbB-1, EC 2.7.10.1, ERBB1, ERBB, HER1, Epidermal
Growth Factor Receptor (Avian Erythroblastic Leukemia Viral (V-Erb-B)
Oncogene Homolog), Erythroblastic Leukemia Viral (V-Erb-B) Oncogene Homolog
(Avian), Avian Erythroblastic Leukemia Viral (V-Erb-B) Oncogene Homolog, Cell
Proliferation-Inducing Protein 61, Cell Growth Inhibiting Protein 40, EC 2.7.10,
NISBD2, PIG61, MENA.
|
Introduction
The epidermal growth factor receptor
(EGF R) subfamily of receptor tyrosine kinases comprises four members: EGF R
(also known as HER1, ErbB1 or ErbB), ErbB2 (Neu, HER-2), ErbB3 (HER-3), and
ErbB4 (HER-4). All family members are type I transmembrane glycoprotein that
has an extracellular domain which contains two cysteine-rich domains separated
by a spacer region that is involved in ligand-binding, and a cytoplasmic domain
which has a membrane-proximal tyrosine kinase domain and a C-terminal tail with
multiple tyrosine autophosphorylation sites. The human EGF R gene encodes a
1210 amino acid (aa) residue precursor with a 24 aa putative signal peptide, a
621 aa extracellular domain, a 23 aa transmembrane domain, and a 542 aa
cytoplasmic domain. EGF R has been shown to bind a subset of the EGF family
ligands, including EGF, amphiregulin, TGFa , betacellulin, epiregulin,
heparin-binding EGF and neuregulin-2 in the absence of a co-receptor. Ligand
binding induces EGF R homodimerization as well as heterdimerization with ErbB2,
resulting in kinase activation, tyrosine phosphorylation and cell signaling.
EGF R can also be recruited to form heterodimers with the ligand-activated
ErbB3 or ErbB4. EGF R signaling has been shown to regulate multiple biological
functions including cell proliferation, differentiation, motility and
apoptosis. In addition, EGF R signaling has also been shown to play a role in
carcinogenesis.
Description
|
EGFR produced in CHO cells
is a single, glycosylated polypeptide chain containing 860 amino acids (25-645
a.a.) and having a molecular mass of 95.5 kDa (Migrates at 100-150 on SDS-PAGE
under reducing conditions).
EGFR is expressed with a
239 amino acid hIgG-His tag at C-Terminus
and purified by proprietary chromatographic techniques.
|
Source
Chinese Hamster Ovary cells.
Physical Appearance
Sterile Filtered colorless solution.
Formulation
|
EGFR protein solution (0.25mg/ml)
contains Phosphate Buffered Saline (pH 7.4) and 10% glycerol. |
Stability
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.?
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).?
Avoid multiple freeze-thaw cycles.
Purity
Greater
than 90.0% as determined by SDS-PAGE.
Amino acid sequence
|
LEEKKVCQGT SNKLTQLGTF
EDHFLSLQRM FNNCEVVLGN LEITYVQRNY DLSFLKTIQE VAGYVLIALN TVERIPLENL QIIRGNMYYE NSYALAVLSN
YDANKTGLKE LPMRNLQEIL HGAVRFSNNP ALCNVESIQW RDIVSSDFLS NMSMDFQNHL GSCQKCDPSC
PNGSCWGAGE ENCQKLTKII CAQQCSGRCR GKSPSDCCHN QCAAGCTGPR ESDCLVCRKF RDEATCKDTC
PPLMLYNPTT YQMDVNPEGK YSFGATCVKK CPRNYVVTDH GSCVRACGAD SYEMEEDGVR KCKKCEGPCR
KVCNGIGIGE FKDSLSINAT NIKHFKNCTS ISGDLHILPV AFRGDSFTHT PPLDPQELDI LKTVKEITGF
LLIQAWPENR TDLHAFENLE IIRGRTKQHG QFSLAVVSLN ITSLGLRSLK EISDGDVIIS GNKNLCYANT INWKKLFGTS
GQKTKIISNR GENSCKATGQ VCHALCSPEG CWGPEPRDCV SCRNVSRGRE CVDKCNLLEG EPREFVENSE
CIQCHPECLP QAMNITCTGR GPDNCIQCAH YIDGPHCVKT CPAGVMGENN TLVWKYADAG HVCHLCHPNC
TYGCTGPGLE GCPTNGPKIP SRSPKSCDKT HTCPPCPAPE LLGGPSVFLF PPKPKDTLMI
SRTPEVTCVV VDVSHEDPEV KFNWYVDGVE VHNAKTKPRE EQYNSTYRVV SVLTVLHQDW LNGKEYKCKV
SNKALPAPIE KTISKAKGQP REPQVYTLPP SRDELTKNQV SLTCLVKGFY PSDIAVEWES NGQPENNYKT
TPPVLDSDGS FFLYSKLTVD KSRWQQGNVF SCSVMHEALH NHYTQKSLSL SPGKHHHHHH.
|
Safety Data Sheet
Usage
ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
