Catalogue number
ENZ-762
Synonyms
Cathepsin W (Lymphopain), LYPN, lymphopain, EC 3.4.22.-.
Introduction
CTSW protein belongs to the peptidase C1 family. CTSW is a cysteine proteinase with a detailed role in the regulation and mechanism of T-cell cytolytic activity. The encoded CTSW is linked to the membrane inside the endoplasmic reticulum of natural killer and cytotoxic T-cells. CTSW expression is up-regulated by interleukin-2.
Description
CTSW Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 378 amino acids (22-376) and having a molecular mass of 42.0 kDa. CTSW is fused to a 23 amino acid His-tag at N-terminus.
Source
Escherichia Coli.
Physical Appearance
Sterile Filtered clear solution.
Formulation
The CTSW solution (0.5mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 0.4M Urea and 10% glycerol.
Stability
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid multiple freeze-thaw cycles.
Purity
Greater than 85% as determined by SDS-PAGE.
Amino acid sequence
MGSSHHHHHH SSGLVPRGSH MGSIRGPLRA QDLGPQPLEL KEAFKLFQIQ FNRSYLSPEE HAHRLDIFAH NLAQAQRLQE EDLGTAEFGV TPFSDLTEEE FGQLYGYRRA AGGVPSMGRE IRSEEPEESV PFSCDWRKVA GAISPIKDQK NCNCCWAMAA AGNIETLWRI SFWDFVDVSV QELLDCGRCG DGCHGGFVWD AFITVLNNSG LASEKDYPFQ GKVRAHRCHP KKYQKVAWIQ DFIMLQNNEH RIAQYLATYG PITVTINMKP LQLYRKGVIK ATPTTCDPQL VDHSVLLVGF GSVKSEEGIW AETVSSQSQP QPPHPTPYWI LKNSWGAQWG EKGYFRLHRG SNTCGITKFP LTARVQKPDM KPRVSCPP.
Safety Data Sheet
Usage
ProSpecs products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
