Catalogue number
ANT-634
Synonyms
Apoptosis-inducing factor 1, mitochondrial, Programmed
cell death protein 8, AIFM1, AIF, PDCD8, CMTX4, COWCK, COXPD6, isoform 2
precursor, Apoptosis-Inducing Factor, Mitochondrion-Associated, 1.
Type
Mouse Anti Human Monoclonal.
Introduction
Apoptosis-Inducing Factor, Mitochondrion-Associated, 1 (AIFM1) is a mitochondrial protein which translocates to the nucleus once apoptosis has been initiated. AIFM1 causes DNA fragmentation and chromatin condensation and also triggers the release of cytochrome c and caspase-9 from mitochondria. Bcl-2 overexpression prevents the release of AIFM1 from mitochondria, but doesn’t block its apoptogenic activity.
Clone
PAT22E9AT.
Physical Appearance
Sterile filtered colorless solution.
Immunogen
Anti-human AIFM1 mAb, is
derived from hybridization of mouse F0 myeloma cells with spleen cells from
BALB/c mice immunized with a recombinant human AIFM1 protein 98-609 amino
acids purified from E. coli.
Ig Subclass
Mouse IgG2a heavy chain and k light chain.
Purification Method
AIFM1 antibody was purified from mouse ascitic fluids by
protein-A affinity chromatography.
Formulation
1mg/ml containing PBS, pH-7.4, 10% Glycerol and 0.02% Sodium Azide.
Storage Procedures
For periods up to 1 month store at 4°C, for longer periods of time, store at -20°C.?
Prevent freeze thaw cycles.
Stability / Shelf Life
12 months at -20°C. 1 month at 4°C.
Applications
The antibody has been tested by ELISA, Western
blot analysis, Flow cytometry and ICC/IF to assure specificity and reactivity.
Since application varies, however, each investigation should be titrated by the
reagent to obtain optimal results.
Safety Data Sheet
Usage
ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
