Catalogue number
ANT-621
Synonyms
Succinate-semialdehyde dehydrogenase mitochondrial,
Aldehyde dehydrogenase family 5 member A1, NAD(+)-dependent succinic
semialdehyde dehydrogenase, ALDH5A1, SSADH, SSDH.
Type
Mouse Anti Human Monoclonal.
Introduction
ALDH5A1 is a mitochondrial NAD(+)-dependent succinic semialdehyde dehydrogenase, which is a member of the aldehyde dehydrogenase family of proteins. The ALDH5A1 protein functions as a mediator to the NADP+-dependent oxidation of aldehydes into acids and has an imperative role in the detoxification of alcohol-derived acetaldehyde, as well as in lipid peroxidation and in the metabolism of corticosteroids, biogenic amines and neurotransmitters. ALDH5A1 is expressed in various tissues, including the liver, heart, lung, brain, kidney and placenta. Deficiency in the ALDH5A1 enzyme, known as 4-hydroxybutyricaciduria, is a rare inborn error in the metabolism of the neurotransmitter 4-aminobutyric acid (GABA). In response to this defect, physiologic fluids from patients accumulate GHB, which is a compound with numerous neuromodulatory properties.
Clone
PAT17F5AT.
Physical Appearance
Sterile filtered colorless solution.
Immunogen
Anti-human ALDH5A1 mAb,
is derived from hybridization of mouse F0 myeloma cells with spleen cells from
BALB/c mice immunized with a recombinant human ALDH5A1
protein 48-535 amino acids purified from
E. coli.
Ig Subclass
Mouse IgG2a heavy chain and k light chain.
Purification Method
ALDH5A1 antibody was purified from mouse ascitic fluids
by protein-A affinity chromatography.
Formulation
1mg/ml containing PBS, pH-7.4, 10% Glycerol and 0.02% Sodium Azide.
Storage Procedures
For periods up to 1 month store at 4°C, for longer periods of time, store at -20°C.?
Prevent freeze thaw cycles.
Stability / Shelf Life
12 months at -20°C. 1 month at 4°C.
Applications
The antibody has been tested by ELISA, Western
blot analysis, Flow cytometry and ICC/IF to assure specificity and reactivity.
Since application varies, however, each investigation should be titrated by the
reagent to obtain optimal results.
Safety Data Sheet
Usage
ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
