Catalogue number
PRO-1462
Synonyms
IS1-RNPL, RNPL, RNA-binding motif protein 3, Putative
RNA-Binding Protein 3, RNA Binding Motif (RNP1, RRM) Protein 3.
Introduction
RBM3
belongs to the glycine-rich RNA-binding protein family and encodes a protein
with one RNA recognition motif (RRM) domain. By
cold shock and low oxygen tension the expression of this gene is induced. On
chromosome 1 a pseudogene exists. Alternate transcriptional splice variants,
encoding different isoforms, have been
characterized. Diseases associated with RBM3
include?vaccinia, andcryptorchidism. GO annotations linked to this gene
include?RNA binding?and?ribosomal large subunit binding. An
important paralog of this gene is?RBMY1J.
Description
RBM3 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 180 amino acids (1-157 a.a) and having a molecular mass of 19kDa.
RBM3 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Source
Escherichia Coli.
Physical Appearance
Sterile Filtered clear solution.
Formulation
RBM3
protein solution (0.25mg/ml) containing 20mM Tris-HCl buffer (pH 8.0), 0.2M
NaCl, 50% glycerol and 5mM DTT.
Stability
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid multiple freeze-thaw cycles.
Purity
Greater than 95.0% as determined by SDS-PAGE.
Amino acid sequence
MGSSHHHHHH SSGLVPRGSH MGSMSSEEGK LFVGGLNFNT DEQALEDHFS SFGPISEVVV VKDRETQRSR
GFGFITFTNP EHASVAMRAM NGESLDGRQI RVDHAGKSAR GTRGGGFGAH GRGRSYSRGG GDQGYGSGRY
YDSRPGGYGY GYGRSRDYNG RNQGGYDRYS GGNYRDNYDN
Safety Data Sheet
Usage
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
